PMID- 29350750 OWN - NLM STAT- MEDLINE DCOM- 20180606 LR - 20220311 IS - 1750-3841 (Electronic) IS - 0022-1147 (Linking) VI - 83 IP - 2 DP - 2018 Feb TI - The Hepatoprotective Effect of Selenium-Enriched Yeast and Gum Arabic Combination on Carbon Tetrachloride-Induced Chronic Liver Injury in Rats. PG - 525-534 LID - 10.1111/1750-3841.14030 [doi] AB - The antioxidant and anti-inflammatory effects of selenium-enriched yeast (SY) and Gum Arabic (GA) have been reported. This study aimed to determine the hepatoprotective effect of SY and GA combination on carbon tetrachloride (CCl(4) )-induced chronic liver injury in rats and to explore their synergistic mechanisms of action. Forty adult male Wistar rats randomly allotted to 5 groups: (A) worked as control, (B) was administered CCl(4) , (C-E) were fed daily by GA, SY, and GA+SY respectively after mixing with basal diet, following CCl(4) -intoxication. GA and SY combination significantly ameliorated CCl(4) -induced reduction in serum total protein with elevation in aspartate transaminase (AST) and alanine transaminase (ALT) in addition to restoring the histopathological changes and hepatic content of hydroxyproline. GA and SY combination was also effective in reducing lipid peroxidation (MDA), consistent with an increase in total antioxidant capacity (T-AOC), glutathione (GSH), superoxide dismutase (SOD) activities, indicating the suppression of liver oxidative stress. Furthermore, liver inflammation was ameliorated by GA and SY combination through inhibition of nuclear factor-kappa (NF-kappaB), tumor necrosis factor-alpha (TNF-alpha), cyclooxygenase-2(COX-2), monocyte chemotactic protein-1 (MCP-1), and toll-like receptor 4(TLR-4) over expression in the liver. Moreover, the up-regulation of proliferating cell nuclear antigen (PCNA) expression by GA and SY combination enhanced the regeneration of liver tissue after CCl(4) -administration. The expression of Collagen1, alpha-smooth muscle actin (alpha-SMA), and transforming growth factor-beta1 (TGFbeta1), was obviously ameliorated by GA and SY combination, suggesting the amelioration of profibrotic response of the liver. Taken together, our current study suggests that GA and SY combination exhibit a significant hepatoprotective activity, which more efficient than GA or SY alone. PRACTICAL APPLICATION: Chronic liver diseases are the serious health problems, which increase the morbidity and mortality in the world today. Selenium-enriched yeast (SY) and Gum Arabic (GA) combination might be potential dietary agents could obviously ameliorate chronic liver damage, higher than GA and SY alone. They act to suppress the inflammation and inhibit the profibrotic response as well as support the liver regeneration. CI - (c) 2018 Institute of Food Technologists(R). FAU - Hamid, Mohammed AU - Hamid M AUID- ORCID: 0000-0001-5242-7901 AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. AD - Coll. of Veterinary Sciences, Univ. of Nyala, Sudan. FAU - Abdulrahim, Yassin AU - Abdulrahim Y AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. AD - Coll. of Veterinary Sciences, Univ. of Nyala, Sudan. FAU - Liu, Dandan AU - Liu D AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. FAU - Qian, Gang AU - Qian G AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. FAU - Khan, Alamzeb AU - Khan A AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. FAU - Huang, Kehe AU - Huang K AD - Coll. of Veterinary Medicine, Nanjing Agricultural Univ., Jiangsu Province, Nanjing 210095, China. AD - Inst. of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural Univ., Nanjing 210095, China. LA - eng PT - Journal Article DEP - 20180119 PL - United States TA - J Food Sci JT - Journal of food science JID - 0014052 RN - 0 (Chemokine CCL2) RN - 0 (NF-kappa B) RN - 0 (Protective Agents) RN - 0 (Toll-Like Receptor 4) RN - 0 (Tumor Necrosis Factor-alpha) RN - 9000-01-5 (Gum Arabic) RN - CL2T97X0V0 (Carbon Tetrachloride) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 2.6.1.1 (Aspartate Aminotransferases) RN - EC 2.6.1.2 (Alanine Transaminase) RN - GAN16C9B8O (Glutathione) RN - H6241UJ22B (Selenium) SB - IM MH - Acacia/chemistry MH - Alanine Transaminase/metabolism MH - Animals MH - Aspartate Aminotransferases/metabolism MH - Carbon Tetrachloride/adverse effects MH - Chemical and Drug Induced Liver Injury/*drug therapy/genetics/metabolism MH - Chemokine CCL2/genetics/metabolism MH - Cyclooxygenase 2/genetics/metabolism MH - Glutathione/metabolism MH - Gum Arabic/*administration & dosage/analysis MH - Humans MH - Lipid Peroxidation/drug effects MH - Liver/drug effects/metabolism MH - Male MH - NF-kappa B/genetics/metabolism MH - Oxidative Stress/drug effects MH - Protective Agents/*administration & dosage MH - Rats MH - Rats, Wistar MH - Saccharomyces cerevisiae/chemistry/metabolism MH - Selenium/*administration & dosage/analysis/metabolism MH - Toll-Like Receptor 4/genetics/metabolism MH - Tumor Necrosis Factor-alpha/genetics/metabolism MH - Up-Regulation OTO - NOTNLM OT - CCl4, chronic liver injury OT - GA and SY combinations OT - liver fibrosis OT - liver inflammation OT - regeneration EDAT- 2018/01/20 06:00 MHDA- 2018/06/07 06:00 CRDT- 2018/01/20 06:00 PHST- 2017/08/06 00:00 [received] PHST- 2017/11/08 00:00 [revised] PHST- 2017/12/07 00:00 [accepted] PHST- 2018/01/20 06:00 [pubmed] PHST- 2018/06/07 06:00 [medline] PHST- 2018/01/20 06:00 [entrez] AID - 10.1111/1750-3841.14030 [doi] PST - ppublish SO - J Food Sci. 2018 Feb;83(2):525-534. doi: 10.1111/1750-3841.14030. Epub 2018 Jan 19.