PMID- 29352747
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20181218
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Linking)
VI  - 19
IP  - 3
DP  - 2018 Mar
TI  - Clathrin and AP1 are required for apical sorting of glycosyl phosphatidyl 
      inositol-anchored proteins in biosynthetic and recycling routes in Madin-Darby 
      canine kidney cells.
PG  - 215-228
LID - 10.1111/tra.12548 [doi]
AB  - Recently, studies in animal models demonstrate potential roles for clathrin and 
      AP1 in apical protein sorting in epithelial tissue. However, the precise 
      functions of these proteins in apical protein transport remain unclear. Here, we 
      reveal mistargeting of endogenous glycosyl phosphatidyl inositol-anchored 
      proteins (GPI-APs) and soluble secretory proteins in Madin-Darby canine kidney 
      (MDCK) cells upon clathrin heavy chain or AP1 subunit knockdown (KD). Using a 
      novel directional endocytosis and recycling assay, we found that these KD cells 
      are not only affected for apical sorting of GPI-APs in biosynthetic pathway but 
      also for their apical recycling and basal-to-apical transcytosis routes. The 
      apical distribution of the t-SNARE syntaxin 3, which is known to be responsible 
      for selective targeting of various apical-destined cargo proteins in both 
      biosynthetic and endocytic routes, is compromised suggesting a molecular 
      explanation for the phenotype in KD cells. Our results demonstrate the importance 
      of biosynthetic and endocytic routes for establishment and maintenance of apical 
      localization of GPI-APs in polarized MDCK cells.
CI  - (c) 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Castillon, Guillaume A
AU  - Castillon GA
AD  - Department of Biochemistry, Sciences II, University of Geneva, Geneva, 
      Switzerland.
FAU - Burriat-Couleru, Patricia
AU  - Burriat-Couleru P
AD  - Department of Biochemistry, Sciences II, University of Geneva, Geneva, 
      Switzerland.
FAU - Abegg, Daniel
AU  - Abegg D
AD  - Department of Biochemistry, Sciences II, University of Geneva, Geneva, 
      Switzerland.
FAU - Criado Santos, Nina
AU  - Criado Santos N
AD  - Department of Biochemistry, Sciences II, University of Geneva, Geneva, 
      Switzerland.
FAU - Watanabe, Reika
AU  - Watanabe R
AUID- ORCID: 0000-0002-7427-7702
AD  - Department of Biochemistry, Sciences II, University of Geneva, Geneva, 
      Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Adaptor Protein Complex 1)
RN  - 0 (CD59 Antigens)
RN  - 0 (Clathrin)
RN  - 0 (Synaptosomal-Associated Protein 25)
SB  - IM
MH  - Adaptor Protein Complex 1/genetics/*metabolism
MH  - Animals
MH  - CD59 Antigens/genetics/*metabolism
MH  - Clathrin/genetics/*metabolism
MH  - Dogs
MH  - Madin Darby Canine Kidney Cells
MH  - Protein Transport
MH  - Synaptosomal-Associated Protein 25/genetics/metabolism
MH  - Transcytosis
OTO - NOTNLM
OT  - GPI-anchored proteins
OT  - MDCK cells
OT  - SNAP technology
OT  - adaptor protein
OT  - apical recycling
OT  - apical sorting
OT  - cell polarity
OT  - clathrin
OT  - endocytosis
OT  - epithelial cells
OT  - polarized sorting
OT  - syntaxin
OT  - transcytosis
EDAT- 2018/01/21 06:00
MHDA- 2018/12/19 06:00
CRDT- 2018/01/21 06:00
PHST- 2017/04/10 00:00 [received]
PHST- 2018/01/16 00:00 [revised]
PHST- 2018/01/16 00:00 [accepted]
PHST- 2018/01/21 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2018/01/21 06:00 [entrez]
AID - 10.1111/tra.12548 [doi]
PST - ppublish
SO  - Traffic. 2018 Mar;19(3):215-228. doi: 10.1111/tra.12548.