PMID- 29354959
OWN - NLM
STAT- MEDLINE
DCOM- 20180702
LR  - 20220409
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 37
IP  - 11
DP  - 2017 Nov 12
TI  - [Effect of acupuncture on macrophage polarization of white adipose tissue in 
      obese mice induced by high-fat diet].
PG  - 1205-11
LID - 10.13703/j.0255-2930.2017.11.018 [doi]
AB  - OBJECTIVE: To observe the effect of acupuncture at acupoints and non-acupoints on 
      macrophage polarization of white adipose tissue in obese mice induced by high-fat 
      diet(HFD). METHODS: Fifty male C57BL/6 mice were randomly divided into a control 
      group (n=10), and other mice were fed with HFD for 16 weeks to establish model. 
      Thirty model mice, which were successfully established and continuously fed with 
      HFD for 8 weeks, were randomized into a model group, a non-acupoint group and an 
      acupuncture group (n=10/group). The mice in the control group were continuously 
      fed with common food for 8 weeks. From the second day after model established, 
      the mice of the acupuncture group were treated with acupuncture at "Guanyuan" (CV 
      4), "Zusanli" (ST 36) and "Weiwanxiashu" (EX-B 3); the mice of the non-acupoint 
      group were treated with acupuncture at two non-acupoints in tail, 15 min a time, 
      once a day for 8 weeks. After model establishment and on the second day after the 
      last acupuncture treatment, the body weight was recorded. The level of serum 
      triglyceride (TG), total cholesterol (TC) in eye socket blood were measured. The 
      morphological structure of epididymis white adipose tissue (eWAT) in mice was 
      observed by hematoxylin and eosin (HE) staining. The mRNA expressions of 
      interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1), tumor necrosis 
      factor alpha (TNF-alpha), IL-10, inducible nitric oxide synthesis (iNOS), CD206 in eWAT 
      of mice were determined with real-time quantitative PCR (RT-qPCR). The protein 
      expressions of iNOS and CD206 in eWAT were determined with immunohistochemistry 
      staining (IHC). RESULTS: Compared with the control group, the body weight in the 
      model, non-acupoint and acupuncture groups increased at the 16th and 24th weeks 
      (all P<0.05). Compared with the model group, the body weight of the acupuncture 
      group at the 24th week decreased (P<0.05). The serum TG and TC in the model group 
      increased compared with those of the control group (both P<0.05). The TC in the 
      non-acupoint group decreased (P<0.05), and the TG and TC in the acupuncture group 
      decreased compared with those in the model group (both P<0.05). Meanwhile, the 
      mRNA expressions of MCP-1, TNF-alpha, IL-6 and iNOS in the model and non-acupoint 
      groups increased compared with those in the control group (all P<0.05), and the 
      mRNA expressions of IL-10 and CD206 decreased (all P<0.05). The mRNA expressions 
      of MCP-1, TNF-alpha, IL-6 and iNOS in the acupuncture group were lower than those in 
      the model group (all P<0.05), and the mRNA expressions of IL-10 and CD206 were 
      higher (both P<0.05). HE staining showed alveolate adipose tissue in the control 
      group with numbers of unilocular adipose cells, vacuolar polygon or circle. The 
      adipose cells in the model group were larger and irregular with larger 
      intercellular space. The adipose cells in the acupuncture group were minified 
      with smaller intercellular space. Compared with those in the control group, the 
      protein expressions of iNOS increased (both P<0.05) and those of CD206 decreased 
      (both P<0.05) in the model and non-acupoint groups. Compared with those in the 
      model group, the protein expression of iNOS decreased (P<0.05) and that of CD206 
      increased (P<0.05) in the acupuncture group. CONCLUSION: Acupuncture can improve 
      inflammation of obese mice through affecting macrophage polarization of white 
      adipose tissue.
FAU - Zhang, Siyi
AU  - Zhang S
AD  - College of Acupuncture and Orthopedics, Hubei University of CM, Wuhan 430061, 
      China.
FAU - Hu, Xia
AU  - Hu X
AD  - Basic Medical College, Hubei University of CM.
FAU - Tang, Hongtu
AU  - Tang H
AD  - College of Acupuncture and Orthopedics, Hubei University of CM; Hubei Provincial 
      Collaborative Innovation Center of Preventive Treatment by Acupuncture and 
      Moxibustion, Wuhan 430061.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
SB  - IM
MH  - Acupuncture Points
MH  - *Acupuncture Therapy
MH  - Adipose Tissue
MH  - Adipose Tissue, White/*cytology
MH  - Animals
MH  - *Cell Polarity
MH  - *Diet, High-Fat
MH  - Epididymis/cytology
MH  - Macrophages/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Obesity/etiology/*pathology
MH  - Random Allocation
OTO - NOTNLM
OT  - acupuncture
OT  - high-fat diet
OT  - macrophage polarization
OT  - obesity
OT  - white adipose
EDAT- 2018/01/23 06:00
MHDA- 2018/07/03 06:00
CRDT- 2018/01/23 06:00
PHST- 2018/01/23 06:00 [entrez]
PHST- 2018/01/23 06:00 [pubmed]
PHST- 2018/07/03 06:00 [medline]
AID - 10.13703/j.0255-2930.2017.11.018 [doi]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2017 Nov 12;37(11):1205-11. doi: 
      10.13703/j.0255-2930.2017.11.018.