PMID- 29355947
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
DP  - 2018 Jan 22
TI  - Corticotropin-releasing hormone-binding protein is up-regulated by brain-derived 
      neurotrophic factor and is secreted in an activity-dependent manner in rat 
      cerebral cortical neurons.
LID - 10.1111/jnc.14310 [doi]
AB  - A recent study revealed that corticotropin-releasing hormone (CRH) in the 
      cerebral cortex (CTX) plays a regulatory role in emotional behaviors in rodents. 
      Given the functional interaction between brain-derived neurotrophic factor (BDNF) 
      and the CRH-signaling pathway in the hypothalamic-pituitary-adrenal axis, we 
      hypothesized that BDNF may regulate gene expression of CRH and its related 
      molecules in the CTX. Findings of real-time quantitative PCR (RT-qPCR) indicated 
      that stimulation of cultured rat cortical neurons with BDNF led to marked 
      elevations in the mRNA levels of CRH and CRH-binding protein (CRH-BP). The 
      BDNF-induced up-regulation of CRH-BP mRNA was attenuated by inhibitors of 
      tropomyosin related kinase (Trk) and MEK, but not by an inhibitor for PI3K and 
      Phospholipase C gamma (PLCgamma). The up-regulation was partially blocked by an 
      inhibitor of lysine-specific demethylase (KDM) 6B. Fluorescent imaging identified 
      the vesicular pattern of pH-sensitive green fluorescent protein-fused CRH-BP 
      (CRH-BP-pHluorin), which co-localized with mCherry-tagged BDNF in cortical 
      neurons. In addition, live-cell imaging detected drastic increases of pHluorin 
      fluorescence in neurites upon membrane depolarization. Finally, we confirmed that 
      tetrodotoxin partially attenuated the BDNF-induced up-regulation of CRH-BP mRNA, 
      but not that of the protein. These observations indicate the following: In 
      cortical neurons, BDNF led to gene expression of CRH-BP and CRH. TrkB, MEK, 
      presumably ERK, and KDM6B are involved in the BDNF-induced gene expression of 
      CRH-BP, and BDNF is able to induce the up-regulation in a neuronal 
      activity-independent manner. It is suggested that CRH-BP is stored into 
      BDNF-containing secretory granules in cortical neurons, and is secreted in 
      response to membrane depolarization.
CI  - (c) 2018 International Society for Neurochemistry.
FAU - Adachi, Naoki
AU  - Adachi N
AD  - Department of Biomedical Chemistry, School of Science and Technology, Kwansei 
      Gakuin University, Sanda, Japan.
FAU - Suzuki, Shingo
AU  - Suzuki S
AUID- ORCID: 0000-0003-3878-1909
AD  - Faculty of Medicine, Department of Anatomy and Neurobiology, Kagawa University, 
      Kagawa, Japan.
FAU - Matsuoka, Hidetada
AU  - Matsuoka H
AD  - School of Medicine, University of Occupational and Environmental Health, 
      Kitakyushu, Japan.
FAU - Fushimi, Satoko
AU  - Fushimi S
AD  - Faculty of Medicine, Department of Anesthesiology, Kagawa University, Kagawa, 
      Japan.
FAU - Ono, Junichiro
AU  - Ono J
AD  - Faculty of Medicine, Department of Anesthesiology, Kagawa University, Kagawa, 
      Japan.
FAU - Ohta, Ken-Ichi
AU  - Ohta KI
AUID- ORCID: 0000-0002-2929-6154
AD  - Faculty of Medicine, Department of Anatomy and Neurobiology, Kagawa University, 
      Kagawa, Japan.
FAU - Hirai, Yohei
AU  - Hirai Y
AD  - Department of Biomedical Chemistry, School of Science and Technology, Kwansei 
      Gakuin University, Sanda, Japan.
FAU - Miki, Takanori
AU  - Miki T
AD  - Faculty of Medicine, Department of Anatomy and Neurobiology, Kagawa University, 
      Kagawa, Japan.
FAU - Koshimizu, Hisatsugu
AU  - Koshimizu H
AUID- ORCID: 0000-0002-8619-6678
AD  - Japan, Society for the Promotion of Science (JSPS), Tokyo, Japan.
AD  - Bio-interface Research Group, Health Research Inst., National Institute of 
      Advanced Industrial Science and Technology (AIST), Ikeda, Japan.
AD  - Division of Systems Medical Science, Institute for Comprehensive Medical Science, 
      Fujita Health University, Toyoake, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180122
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
OTO - NOTNLM
OT  - BDNF
OT  - CRH
OT  - CRH-BP
OT  - TrkB
OT  - cerebral cortical neurons
EDAT- 2018/01/23 06:00
MHDA- 2018/01/23 06:00
CRDT- 2018/01/23 06:00
PHST- 2017/08/04 00:00 [received]
PHST- 2017/12/19 00:00 [revised]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/01/23 06:00 [pubmed]
PHST- 2018/01/23 06:00 [medline]
PHST- 2018/01/23 06:00 [entrez]
AID - 10.1111/jnc.14310 [doi]
PST - aheadofprint
SO  - J Neurochem. 2018 Jan 22. doi: 10.1111/jnc.14310.