PMID- 29356574
OWN - NLM
STAT- MEDLINE
DCOM- 20180417
LR  - 20180417
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 37
IP  - 3
DP  - 2018 Mar
TI  - Pyruvate Kinase M2 Modulates the Glycolysis of Chondrocyte and Extracellular 
      Matrix in Osteoarthritis.
PG  - 271-277
LID - 10.1089/dna.2017.4048 [doi]
AB  - Pyruvate kinase M2 (PKM2) has been wildly verified to modulate glycolysis in 
      tumor cells. However, the role of PKM2 on the glycolysis of osteoarthritis (OA) 
      chondrocytes is still unclear. In present study, we investigate the function of 
      PKM2 on OA chondrocyte glycolysis and the collagen matrix generation in vitro. 
      Results showed that PKM2 was upregulated in OA chondrocytes compared with healthy 
      control chondrocytes. In OA chondrocytes, ATP expression was lower compared with 
      healthy control chondrocytes. Loss-of-function experiment showed that PKM2 
      knockdown mediated by lentivirus transfection could significantly suppress the 
      glucose consumption and lactate secretion levels and decrease glucose 
      transporter-1 (Glut-1), lactate dehydrogenase A (LDHA), and hypoxia inducible 
      factor 1-alpha (HIF-1alpha), indicating the inhibition of PKM2 knockdown on 
      glycolysis. Moreover, Cell Counting Kit-8 (CCK-8), flow cytometry, and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay showed 
      that PKM2 knockdown inhibited OA chondrocyte proliferation and promoted the 
      apoptosis. Western blot and immunocytochemical staining showed that PKM2 
      knockdown downregulated the expression levels of COL2A1 and SOX-9. In summary, 
      our results conclude that PKM2 modulates the glycolysis and extracellular matrix 
      generation, providing the vital role of PKM2 on OA pathogenesis and a novel 
      therapeutic target for OA.
FAU - Yang, Xiaobo
AU  - Yang X
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Chen, Weiping
AU  - Chen W
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Zhao, Xiang
AU  - Zhao X
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Chen, Linwei
AU  - Chen L
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Li, Wanli
AU  - Li W
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Ran, Jisheng
AU  - Ran J
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
FAU - Wu, Lidong
AU  - Wu L
AD  - The Second Affiliated Hospital of Zhejiang University, School of Medicine, 
      Hangzhou, China .
LA  - eng
PT  - Journal Article
DEP - 20180122
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Adult
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chondrocytes/*enzymology
MH  - Enzyme Induction
MH  - Extracellular Matrix/*enzymology
MH  - *Glycolysis
MH  - Humans
MH  - Middle Aged
MH  - Osteoarthritis, Knee/*enzymology/pathology
MH  - Pyruvate Kinase/*physiology
MH  - Up-Regulation
OTO - NOTNLM
OT  - PKM2
OT  - chondrocyte
OT  - extracellular matrix
OT  - glycolysis
OT  - osteoarthritis
EDAT- 2018/01/23 06:00
MHDA- 2018/04/18 06:00
CRDT- 2018/01/23 06:00
PHST- 2018/01/23 06:00 [pubmed]
PHST- 2018/04/18 06:00 [medline]
PHST- 2018/01/23 06:00 [entrez]
AID - 10.1089/dna.2017.4048 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2018 Mar;37(3):271-277. doi: 10.1089/dna.2017.4048. Epub 2018 Jan 
      22.