PMID- 29356894
OWN - NLM
STAT- MEDLINE
DCOM- 20180821
LR  - 20220331
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 247
IP  - 5
DP  - 2018 May
TI  - Development of Thinopyrum ponticum-specific molecular markers and FISH probes 
      based on SLAF-seq technology.
PG  - 1099-1108
LID - 10.1007/s00425-018-2845-6 [doi]
AB  - Based on SLAF-seq, 67 Thinopyrum ponticum-specific markers and eight Th. 
      ponticum-specific FISH probes were developed, and these markers and probes could 
      be used for detection of alien chromatin in a wheat background. Decaploid 
      Thinopyrum ponticum (2n = 10x = 70) is a valuable gene reservoir for wheat 
      improvement. Identification of Th. ponticum introgression would facilitate its 
      transfer into diverse wheat genetic backgrounds and its practical utilization in 
      wheat improvement. Based on specific-locus-amplified fragment sequencing 
      (SLAF-seq) technology, 67 new Th. ponticum-specific molecular markers and eight 
      Th. ponticum-specific fluorescence in situ hybridization (FISH) probes have been 
      developed from a tiny wheat-Th. ponticum translocation line. These newly 
      developed molecular markers allowed the detection of Th. ponticum DNA in a 
      variety of materials specifically and steadily at high throughput. According to 
      the hybridization signal pattern, the eight Th. ponticum-specific probes could be 
      divided into two groups. The first group including five dispersed repetitive 
      sequence probes could identify Th. ponticum chromatin more sensitively and 
      accurately than genomic in situ hybridization (GISH). Whereas the second group 
      having three tandem repetitive sequence probes enabled the discrimination of Th. 
      ponticum chromosomes together with another clone pAs1 in wheat-Th. ponticum 
      partial amphiploid Xiaoyan 68.
FAU - Liu, Liqin
AU  - Liu L
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
FAU - Luo, Qiaoling
AU  - Luo Q
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Teng, Wan
AU  - Teng W
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
FAU - Li, Bin
AU  - Li B
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
FAU - Li, Hongwei
AU  - Li H
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
FAU - Li, Yiwen
AU  - Li Y
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China.
FAU - Li, Zhensheng
AU  - Li Z
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China. zsli@genetics.ac.cn.
FAU - Zheng, Qi
AU  - Zheng Q
AD  - Institute of Genetics and Developmental Biology, Chinese Academy of 
      Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering, Beijing, 
      100101, China. qzheng@genetics.ac.cn.
LA  - eng
GR  - XDA08030105/the Strategic Priority Research Program of the Chinese Academy of 
      Sciences/
GR  - 2016YFD0102000/the National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20180122
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Chromatin)
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromatin/genetics
MH  - DNA, Plant/genetics
MH  - Genetic Markers/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Amplification Techniques/methods
MH  - Poaceae/*genetics
MH  - Sequence Analysis, DNA/methods
MH  - Triticum/genetics
OTO - NOTNLM
OT  - FISH
OT  - GISH
OT  - Molecular markers
OT  - SLAF-seq
OT  - Thinopyrum ponticum
OT  - Triticum aestivum
EDAT- 2018/01/23 06:00
MHDA- 2018/08/22 06:00
CRDT- 2018/01/23 06:00
PHST- 2017/10/17 00:00 [received]
PHST- 2018/01/07 00:00 [accepted]
PHST- 2018/01/23 06:00 [pubmed]
PHST- 2018/08/22 06:00 [medline]
PHST- 2018/01/23 06:00 [entrez]
AID - 10.1007/s00425-018-2845-6 [pii]
AID - 10.1007/s00425-018-2845-6 [doi]
PST - ppublish
SO  - Planta. 2018 May;247(5):1099-1108. doi: 10.1007/s00425-018-2845-6. Epub 2018 Jan 
      22.