PMID- 29357727
OWN - NLM
STAT- MEDLINE
DCOM- 20191014
LR  - 20220317
IS  - 1476-4954 (Electronic)
IS  - 1476-4954 (Linking)
VI  - 32
IP  - 14
DP  - 2019 Jul
TI  - Amniotic fluid HIF1alpha and exosomal HIF1alpha in cervical insufficiency patients with 
      physical examination-indicated cerclage.
PG  - 2287-2294
LID - 10.1080/14767058.2018.1432037 [doi]
AB  - OBJECTIVE: Hypoxia inducible factor 1alpha (HIF1alpha) has been reported to activate 
      inflammatory cascade. Recently, exosomes have been known to have pivotal roles in 
      intercellular communication. The aim of this study was to compare the 
      concentration of amniotic fluid (AF) HIF1alpha, exosomal HIF1alpha, and inflammatory 
      cytokines such as interleukin 1alpha (IL1alpha), interleukin 1beta (IL1beta), interleukin 6 
      (IL6), and tumor necrosis factor alpha (TNFalpha) between physical examination-indicated 
      cerclage (PEIC) and control group. We also investigated the associations between 
      biomarkers and amniocentesis-to-delivery interval and the correlations of 
      inflammatory cytokines, HIF1alpha, and exosomal HIF1alpha. METHODS: Case-control study 
      was performed. Cases are defined as 16 patients who underwent PEIC and controls 
      are 19 women who underwent amniocentesis for confirming chromosomal 
      abnormalities. The concentration of IL1alpha, IL1beta, IL6, TNFalpha, HIF1alpha, and exosomal 
      HIF1alpha were measured using enzyme-linked immunosorbent assay (ELISA). Exosomes 
      were confirmed by tumor susceptibility Gene 101 (TSG 101) and transmission 
      electron microscopy (TEM). RESULTS: The mean HIF1alpha in PEIC group was higher than 
      control group (PEIC, 15.03 +/- 9.60-pg/mL versus control, 2.96 +/- 1.99 pg/mL; 
      p < .01). There were significant differences in inflammatory cytokines between 
      two groups. A significant difference in exosomal HIF1alpha was shown between two 
      groups (PEIC, 27.97 +/- 28.61-microg/mL versus control, 12.42 +/- 8.20 microg/mL; p < .01). 
      HIF1alpha, IL1alpha, IL6, TNFalpha, and exosomal HIF1alpha showed significantly negative 
      association with cerclage-to-delivery interval. However, IL1beta was not associated 
      with cerclage-to-delivery interval. HIF1alpha was positively correlated with exosomal 
      HIF1alpha (rho = 0.93, p < .01). Both HIF1alpha and exosomal HIF1alpha were significantly 
      associated with TNFalpha (rho = 0.94, p < .01; rho = 0.97, p < .01). Both HIF-1alpha and 
      exosomal HIF1alpha had positive correlation with IL1alpha (rho = 0.96, p < .01; 
      rho = 0.91, p < .01). However, IL1beta showed no correlations with HIF1alpha and 
      exosomal HIF1alpha. A positive correlation between HIF-1alpha and IL6 was observed 
      (rho = 0.58, p = .01.) Exosomal HIF1alpha also had correlation with IL6 (rho = 0.52, 
      p = .03). CONCLUSIONS: This study demonstrated that amniotic fluid (AF) HIF1alpha and 
      AF exosomal HIF1alpha were higher in physical examination-indicated cerclage (PEIC) 
      group than control group. AF HIF1alpha and AF exosomal HIF1alpha were associated with 
      shorter amniocentesis-to-delivery interval. More importantly, they had positive 
      correlations with AF inflammatory cytokines such as IL1alpha, IL6, and TNFalpha. Our 
      results may indicate that AF HIF1alpha and AF exosomes interact with AF inflammatory 
      cytokines and contribute inflammatory cascade in complicated pregnancies.
FAU - Song, Ji Eun
AU  - Song JE
AD  - a Department of Obstetrics and Gynecology , Hallym University School of Medicine 
      , Seoul , Republic of Korea.
FAU - Park, Seok Ju
AU  - Park SJ
AD  - b Ilsong Institute of Life Science , Hallym University , Anyang , Republic of 
      Korea.
FAU - Lee, Keun Young
AU  - Lee KY
AD  - a Department of Obstetrics and Gynecology , Hallym University School of Medicine 
      , Seoul , Republic of Korea.
FAU - Lee, Wang Jae
AU  - Lee WJ
AD  - c Department of Anatomy , Seoul National University College of Medicine , Seoul , 
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20180215
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Amniocentesis/statistics & numerical data
MH  - Amniotic Fluid/*metabolism
MH  - Case-Control Studies
MH  - Cerclage, Cervical
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exosomes/metabolism
MH  - Female
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*analysis
MH  - Infant, Newborn
MH  - Interleukin-1beta/analysis
MH  - Interleukin-6/analysis
MH  - Pregnancy
MH  - Premature Birth
MH  - Retrospective Studies
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Uterine Cervical Incompetence/*metabolism/surgery
OTO - NOTNLM
OT  - Amniotic fluid
OT  - exosome
OT  - hypoxia inducible factor 1alpha
OT  - interleukin 1alpha
OT  - interleukin 1beta
OT  - interleukin 6
OT  - physical examination-indicated cerclage
OT  - tumor necrosis factor alpha
EDAT- 2018/01/24 06:00
MHDA- 2019/10/15 06:00
CRDT- 2018/01/24 06:00
PHST- 2018/01/24 06:00 [pubmed]
PHST- 2019/10/15 06:00 [medline]
PHST- 2018/01/24 06:00 [entrez]
AID - 10.1080/14767058.2018.1432037 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2019 Jul;32(14):2287-2294. doi: 
      10.1080/14767058.2018.1432037. Epub 2018 Feb 15.