PMID- 29357945
OWN - NLM
STAT- MEDLINE
DCOM- 20180813
LR  - 20181113
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 11
IP  - 1
DP  - 2018 Jan 22
TI  - Human stem cells express pannexins.
PG  - 54
LID - 10.1186/s13104-018-3125-z [doi]
LID - 54
AB  - OBJECTIVE: Pannexins are channel proteins important for the release of calcium 
      and adenosine triphosphate, which are among other functions involved in early 
      development. Here, the expression of pannexins was investigated in induced 
      pluripotent stem cells derived from human cord blood endothelial cells (hCBiPS2), 
      in hematopoietic stem cell-derived induced pluripotent stem cells 
      (HSC_F1285_T-iPS2) and in human embryonic stem cells (HES-3). The expression of 
      pannexin (Panx) 1-3 mRNAs was analyzed in all three undifferentiated stem cell 
      lines. Stem cells then underwent undirected differentiation into embryoid bodies 
      and were analyzed regarding expression of germ layer-specific genes. RESULTS: 
      Panx1, Panx2, and Panx3 mRNAs were expressed in all undifferentiated stem cell 
      lines investigated. In comparison, Panx1 showed the highest expression among all 
      pannexins. The undirected differentiation resulted in a mixed germ layer genotype 
      in all three stem cell lines. Whereas the expression of Panx1 was not affected by 
      differentiation, the expression of Panx2 was slightly increased in differentiated 
      hCBiPS2 cells, HSC_F1285_T-iPS2 as well as HES3 cells as compared to their 
      undifferentiated counterparts. A slight increase of Panx3 expression was observed 
      in differentiated hCBiPS2 cells only. In conclusion, pluripotent stem cells 
      express all three pannexin genes.
FAU - Hainz, Nadine
AU  - Hainz N
AD  - Department of Anatomy and Cell Biology, Saarland University, Kirrberger Strasse, 
      Building 61, Saar, 66421, Homburg, Germany.
FAU - Beckmann, Anja
AU  - Beckmann A
AD  - Department of Anatomy and Cell Biology, Saarland University, Kirrberger Strasse, 
      Building 61, Saar, 66421, Homburg, Germany.
FAU - Schubert, Madline
AU  - Schubert M
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplant and Vascular Surgery, Hannover 
      Medical School, 30625, Hannover, Germany.
AD  - REBIRTH-Cluster of Excellence, Hannover Medical School, 30625, Hannover, Germany.
FAU - Haase, Alexandra
AU  - Haase A
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplant and Vascular Surgery, Hannover 
      Medical School, 30625, Hannover, Germany.
AD  - REBIRTH-Cluster of Excellence, Hannover Medical School, 30625, Hannover, Germany.
FAU - Martin, Ulrich
AU  - Martin U
AD  - Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), 
      Department of Cardiac, Thoracic, Transplant and Vascular Surgery, Hannover 
      Medical School, 30625, Hannover, Germany.
AD  - REBIRTH-Cluster of Excellence, Hannover Medical School, 30625, Hannover, Germany.
FAU - Tschernig, Thomas
AU  - Tschernig T
AUID- ORCID: 0000-0002-7788-1796
AD  - Department of Anatomy and Cell Biology, Saarland University, Kirrberger Strasse, 
      Building 61, Saar, 66421, Homburg, Germany. thomas.tschernig@uks.eu.
FAU - Meier, Carola
AU  - Meier C
AD  - Department of Anatomy and Cell Biology, Saarland University, Kirrberger Strasse, 
      Building 61, Saar, 66421, Homburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180122
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (PANX1 protein, human)
RN  - 0 (PANX2 protein, human)
RN  - 0 (PANX3 protein, human)
SB  - IM
MH  - Cell Line
MH  - Connexins/*genetics
MH  - Endothelial Progenitor Cells/cytology/metabolism
MH  - Fetal Blood/cytology
MH  - *Gene Expression
MH  - Human Embryonic Stem Cells/cytology/metabolism
MH  - Humans
MH  - Induced Pluripotent Stem Cells/cytology/metabolism
MH  - Nerve Tissue Proteins/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/cytology/*metabolism
PMC - PMC5778636
OTO - NOTNLM
OT  - Differentiation
OT  - Endoderm
OT  - Human stem cells
OT  - Pannexins
OT  - Regulation
EDAT- 2018/01/24 06:00
MHDA- 2018/08/14 06:00
PMCR- 2018/01/22
CRDT- 2018/01/24 06:00
PHST- 2017/11/03 00:00 [received]
PHST- 2018/01/03 00:00 [accepted]
PHST- 2018/01/24 06:00 [entrez]
PHST- 2018/01/24 06:00 [pubmed]
PHST- 2018/08/14 06:00 [medline]
PHST- 2018/01/22 00:00 [pmc-release]
AID - 10.1186/s13104-018-3125-z [pii]
AID - 3125 [pii]
AID - 10.1186/s13104-018-3125-z [doi]
PST - epublish
SO  - BMC Res Notes. 2018 Jan 22;11(1):54. doi: 10.1186/s13104-018-3125-z.