PMID- 29358665
OWN - NLM
STAT- MEDLINE
DCOM- 20181123
LR  - 20190122
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Jan 22
TI  - Anaerobic methanotrophic communities thrive in deep submarine permafrost.
PG  - 1291
LID - 10.1038/s41598-018-19505-9 [doi]
LID - 1291
AB  - Thawing submarine permafrost is a source of methane to the subsurface biosphere. 
      Methane oxidation in submarine permafrost sediments has been proposed, but the 
      responsible microorganisms remain uncharacterized. We analyzed archaeal 
      communities and identified distinct anaerobic methanotrophic assemblages of 
      marine and terrestrial origin (ANME-2a/b, ANME-2d) both in frozen and completely 
      thawed submarine permafrost sediments. Besides archaea potentially involved in 
      anaerobic oxidation of methane (AOM) we found a large diversity of archaea mainly 
      belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane 
      concentrations and delta(13)C-methane signatures distinguish horizons of potential 
      AOM coupled either to sulfate reduction in a sulfate-methane transition zone 
      (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese 
      or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ 
      hybridization (FISH) corroborate potential activity of AOM communities in 
      submarine permafrost sediments at low temperatures. Modeled potential AOM 
      consumes 72-100% of submarine permafrost methane and up to 1.2 Tg of carbon per 
      year for the total expected area of submarine permafrost. This is comparable with 
      AOM habitats such as cold seeps. We thus propose that AOM is active where 
      submarine permafrost thaws, which should be included in global methane budgets.
FAU - Winkel, Matthias
AU  - Winkel M
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany. mwinkel@gfz-potsdam.de.
FAU - Mitzscherling, Julia
AU  - Mitzscherling J
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany.
FAU - Overduin, Pier P
AU  - Overduin PP
AD  - Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, 
      Periglacial Research, 14473, Potsdam, Germany.
FAU - Horn, Fabian
AU  - Horn F
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany.
FAU - Winterfeld, Maria
AU  - Winterfeld M
AD  - Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Marine 
      Geochemistry, 27570, Bremerhaven, Germany.
FAU - Rijkers, Ruud
AU  - Rijkers R
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany.
FAU - Grigoriev, Mikhail N
AU  - Grigoriev MN
AD  - Mel'nikov Permafrost Institute, SB RAS, Yakutsk, 677010, Russia.
FAU - Knoblauch, Christian
AU  - Knoblauch C
AUID- ORCID: 0000-0002-7147-1008
AD  - Institute of Soil Science, Universitat Hamburg, 20146, Hamburg, Germany.
FAU - Mangelsdorf, Kai
AU  - Mangelsdorf K
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 3.2 
      Organic Geochemistry, 14473, Potsdam, Germany.
FAU - Wagner, Dirk
AU  - Wagner D
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany.
FAU - Liebner, Susanne
AU  - Liebner S
AD  - GFZ German Research Centre for Geosciences, Helmholtz Centre Potsdam, Section 5.3 
      Geomicrobiology, 14473, Potsdam, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180122
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Archaeal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7440-44-0 (Carbon)
RN  - N762921K75 (Nitrogen)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis/*physiology
MH  - Archaea/classification/genetics/isolation & purification/*metabolism
MH  - Arctic Regions
MH  - Biodiversity
MH  - Carbon/metabolism
MH  - DNA, Archaeal/*genetics
MH  - Geologic Sediments/microbiology
MH  - Methane/*metabolism
MH  - Nitrogen/metabolism
MH  - Oceans and Seas
MH  - Oxidation-Reduction
MH  - Permafrost/*microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Russia
PMC - PMC5778128
COIS- The authors declare that they have no competing interests.
EDAT- 2018/01/24 06:00
MHDA- 2018/11/24 06:00
PMCR- 2018/01/22
CRDT- 2018/01/24 06:00
PHST- 2017/09/12 00:00 [received]
PHST- 2017/12/22 00:00 [accepted]
PHST- 2018/01/24 06:00 [entrez]
PHST- 2018/01/24 06:00 [pubmed]
PHST- 2018/11/24 06:00 [medline]
PHST- 2018/01/22 00:00 [pmc-release]
AID - 10.1038/s41598-018-19505-9 [pii]
AID - 19505 [pii]
AID - 10.1038/s41598-018-19505-9 [doi]
PST - epublish
SO  - Sci Rep. 2018 Jan 22;8(1):1291. doi: 10.1038/s41598-018-19505-9.