PMID- 29358954
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220311
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2017
DP  - 2017
TI  - The Role of Nephronectin on Proliferation and Differentiation in Human Dental 
      Pulp Stem Cells.
PG  - 2546261
LID - 10.1155/2017/2546261 [doi]
LID - 2546261
AB  - AIM: The purpose of the current study was to investigate the effects of 
      nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). METHODOLOGY: Npnt 
      was coated to nontissue culture-treated polystyrene (non-PS) plates. The presence 
      of immobilized protein on the surface was detected by polyclonal rabbit primary 
      anti-Npnt antibody. Then the cell number was counted and compared with PBS-, 
      bovine serum albumin- (BSA-), fish scale type I collagen- (FCOL1-), and human 
      fibronectin- (Fn-) coated wells. Cell proliferation was assessed using CCK-8 
      assay. Cell morphology was observed under light microscopy and fluorescence 
      microscopy. Lastly, the mRNA expression profiles of integrins, dentin 
      sialophosphoprotein (DSPP), bone sialoprotein (BSP), and mineralization capacity 
      of hDPSCs were investigated by real time RT-PCR and alizarin red staining, 
      respectively. RESULTS: Npnt mediates hDPSC adhesion and spreading partially via 
      the Arg-Gly-Asp (RGD) motif. Npnt enhanced the mRNA expression of ITGA1, ITGA4, 
      ITGA7, and ITGB1 on day five. Npnt downregulated DSPP but significantly 
      upregulated BSP mRNA expression at day 28. Further, Npnt and FCOL1 accelerated 
      the matrix mineralization in hDPSCs. CONCLUSIONS: The current findings implicate 
      that Npnt would be favorable to recruit hDPSCs and conducive to mineralization in 
      hDPSCs. The combination of Npnt with hDPSCs may offer a promising approach for 
      hard tissue regeneration.
FAU - Tang, Jia
AU  - Tang J
AUID- ORCID: 0000-0002-5025-2674
AD  - Division of Biochemistry, Department of Oral Biology, School of Dentistry, Health 
      Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 
      061-0293, Japan.
FAU - Saito, Takashi
AU  - Saito T
AUID- ORCID: 0000-0001-8816-7187
AD  - Division of Clinical Cariology and Endodontology, Department of Oral 
      Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, 1757 
      Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
LA  - eng
PT  - Journal Article
DEP - 20171119
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5735320
EDAT- 2018/01/24 06:00
MHDA- 2018/01/24 06:01
PMCR- 2017/11/19
CRDT- 2018/01/24 06:00
PHST- 2017/06/05 00:00 [received]
PHST- 2017/09/28 00:00 [revised]
PHST- 2017/10/16 00:00 [accepted]
PHST- 2018/01/24 06:00 [entrez]
PHST- 2018/01/24 06:00 [pubmed]
PHST- 2018/01/24 06:01 [medline]
PHST- 2017/11/19 00:00 [pmc-release]
AID - 10.1155/2017/2546261 [doi]
PST - ppublish
SO  - Stem Cells Int. 2017;2017:2546261. doi: 10.1155/2017/2546261. Epub 2017 Nov 19.