PMID- 29360450
OWN - NLM
STAT- MEDLINE
DCOM- 20180222
LR  - 20220331
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 496
IP  - 3
DP  - 2018 Feb 12
TI  - Noninvasive, targeted gene therapy for acute spinal cord injury using 
      LIFU-mediated BDNF-loaded cationic nanobubble destruction.
PG  - 911-920
LID - S0006-291X(18)30138-4 [pii]
LID - 10.1016/j.bbrc.2018.01.123 [doi]
AB  - Various gene delivery systems have been widely studied for the acute spinal cord 
      injury (SCI) treatment. In the present study, a novel type of brain-derived 
      neurotrophic factor (BDNF)-loaded cationic nanobubbles (CNBs) conjugated with 
      MAP-2 antibody (mAb(MAP-2)/BDNF/CNBs) was prepared to provide low-intensity 
      focused ultrasound (LIFU)-targeted gene therapy. In vitro experiments, the 
      ultrasound-targeted tranfection to BDNF overexpressioin in neurons and 
      efficiently inhibition neuronal apoptosis have been demonstrated, and the 
      elaborately designed mAb(MAP-2)/BDNF/CNBs can specifically target to the neurons. 
      Furthermore, in a acute SCI rat model, LIFU-mediated mAb(MAP-2)/BDNF/CNBs 
      transfection significantly increased BDNF expression, attenuated histological 
      injury, decreased neurons loss, inhibited neuronal apoptosis in injured spinal 
      cords, and increased BBB scores in SCI rats. LIFU-mediated mAb(MAP-2)/BDNF/CNBs 
      destruction significantly increase transfection efficiency of BDNF gene both 
      in vitro and in vivo, and has a significant neuroprotective effect on the injured 
      spinal cord. Therefore, the combination of LIFU irradiation and gene therapy 
      through mAb(MAP-2)/BDNF/CNBs can be considered as a novel non-invasive and 
      targeted treatment for gene therapy of SCI.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Song, Zhaojun
AU  - Song Z
AD  - Department of Orthopaedics, Suining Central Hospital, Sichuan, People's Republic 
      of China.
FAU - Ye, Yongjie
AU  - Ye Y
AD  - Department of Orthopaedics, Suining Central Hospital, Sichuan, People's Republic 
      of China.
FAU - Zhang, Zhi
AU  - Zhang Z
AD  - Department of Orthopaedics, Suining Central Hospital, Sichuan, People's Republic 
      of China. Electronic address: zhangzhispine@163.net.
FAU - Shen, Jieliang
AU  - Shen J
AD  - Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, People's Republic of China.
FAU - Hu, Zhenming
AU  - Hu Z
AD  - Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, People's Republic of China.
FAU - Wang, Zhigang
AU  - Wang Z
AD  - Institution of Ultrasound Imaging, The Second Affiliated Hospital, Chongqing 
      Medical University, Chongqing, People's Republic of China.
FAU - Zheng, Jiazhuang
AU  - Zheng J
AD  - Department of Orthopaedics, Suining Central Hospital, Sichuan, People's Republic 
      of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180131
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cations)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Fluorocarbons)
RN  - 0 (Nanocapsules)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - CK0N3WH0SR (perflutren)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage/genetics
MH  - Cations
MH  - Delayed-Action Preparations/*administration & dosage
MH  - Fluorocarbons/radiation effects
MH  - Gene Targeting/methods
MH  - Genetic Therapy/*methods
MH  - High-Energy Shock Waves
MH  - Male
MH  - Molecular Targeted Therapy/methods
MH  - Nanocapsules/chemistry/*radiation effects
MH  - Nanospheres/chemistry/radiation effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sonication/*methods
MH  - Spinal Cord Injuries/*genetics/pathology/*therapy
MH  - Treatment Outcome
OTO - NOTNLM
OT  - BDNF
OT  - Cationic nanobubble
OT  - Gene therapy
OT  - LIFU
OT  - Spinal cord injury
EDAT- 2018/01/24 06:00
MHDA- 2018/02/23 06:00
CRDT- 2018/01/24 06:00
PHST- 2018/01/17 00:00 [received]
PHST- 2018/01/19 00:00 [accepted]
PHST- 2018/01/24 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
PHST- 2018/01/24 06:00 [entrez]
AID - S0006-291X(18)30138-4 [pii]
AID - 10.1016/j.bbrc.2018.01.123 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Feb 12;496(3):911-920. doi: 
      10.1016/j.bbrc.2018.01.123. Epub 2018 Jan 31.