PMID- 29362854
OWN - NLM
STAT- MEDLINE
DCOM- 20180502
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 410
IP  - 7
DP  - 2018 Mar
TI  - Simultaneous quantification of endogenous and exogenous plasma glucose by isotope 
      dilution LC-MS/MS with indirect MRM of the derivative tag.
PG  - 2011-2018
LID - 10.1007/s00216-018-0872-6 [doi]
AB  - Quantification of endogenous and exogenous plasma glucose can help more 
      comprehensively evaluate the glucose metabolic status. A ratio-based approach 
      using isotope dilution liquid chromatography tandem mass spectrometry (ID 
      LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag 
      was developed to simultaneously quantify endo-/exogenous plasma glucose. Using 
      diluted D-[(13)C(6)] glucose as tracer of exogenous glucose, (12)C(6)/(13)C(6) 
      glucoses were first derivatized and then data were acquired in MRM mode. The 
      metabolism of exogenous glucose can be tracked and the concentration ratio of 
      endo/exo-genous glucose can be measured by calculating the endo-/exo-genous 
      glucose concentrations from peak area ratio of specific daughter ions. Joint 
      application of selective derivatization and MRM analysis not only improves the 
      sensitivity but also minimizes the interference from the background of plasma, 
      which warrants the accuracy and reproducibility. Good agreement between the 
      theoretical and calculated concentration ratios was obtained with a linear 
      correlation coefficient (R) of 0.9969 in the range of D-glucose from 0.5 to 
      20.0 mM, which covers the healthy and diabetic physiological scenarios. 
      Satisfactory reproducibility was obtained by evaluation of the intra- and 
      inter-day precisions with relative standard deviations (RSDs) less than 5.16%, 
      and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high 
      concentration, respectively. The method was successfully applied to simultaneous 
      determination of the endo-/exogenous glucose concentration in plasma of 
      non-diabetic and type II diabetic cynomolgus monkeys. Graphical Abstract The 
      scheme of the proposed ratio-based approach using isotope dilution LC-MS/MS with 
      indirect MRM of the derivative tag for simultaneous quantification of endogenous 
      and exogenous plasma glucose.
FAU - Yu, Lingling
AU  - Yu L
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, 
      Kunming, Yunnan, 650201, China.
AD  - School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, 
      650091, China.
FAU - Wen, Chao
AU  - Wen C
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, 
      Kunming, Yunnan, 650201, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Li, Xing
AU  - Li X
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, 
      Kunming, Yunnan, 650201, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Fang, Shiqi
AU  - Fang S
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, 
      Kunming, Yunnan, 650201, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Yang, Lichuan
AU  - Yang L
AD  - Kunming Biomed International, Kunming, Yunnan, 650500, China.
FAU - Wang, Tony
AU  - Wang T
AD  - Kunming Biomed International, Kunming, Yunnan, 650500, China.
FAU - Hu, Kaifeng
AU  - Hu K
AUID- ORCID: 0000-0002-2343-2012
AD  - State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming 
      Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Heilongtan, 
      Kunming, Yunnan, 650201, China. kaifenghu@mail.kib.ac.cn.
LA  - eng
GR  - 2017YFC0906900/National key R & D program of China/
GR  - 2012HA015/Yunnan Provincial Science and Technology Department/
GR  - 21505142/National Natural Science Foundation of China/
PT  - Journal Article
PT  - Validation Study
DEP - 20180123
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Blood Glucose)
RN  - 0 (Carbon Isotopes)
SB  - IM
MH  - Animals
MH  - Blood Glucose/*analysis/metabolism
MH  - Carbon Isotopes/analysis/metabolism
MH  - Chromatography, Liquid/methods
MH  - Diabetes Mellitus/*blood/metabolism
MH  - Humans
MH  - Indicator Dilution Techniques
MH  - Limit of Detection
MH  - Macaca fascicularis
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Derivatization
OT  - Diabetes
OT  - Endogenous glucose
OT  - Exogenous glucose
OT  - LC-MS/MS
EDAT- 2018/01/25 06:00
MHDA- 2018/05/03 06:00
CRDT- 2018/01/25 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2018/01/11 00:00 [accepted]
PHST- 2017/12/23 00:00 [revised]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2018/05/03 06:00 [medline]
PHST- 2018/01/25 06:00 [entrez]
AID - 10.1007/s00216-018-0872-6 [pii]
AID - 10.1007/s00216-018-0872-6 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2018 Mar;410(7):2011-2018. doi: 10.1007/s00216-018-0872-6. 
      Epub 2018 Jan 23.