PMID- 29363728
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20211022
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 3
DP  - 2018 Mar
TI  - Interleukin-17 induces angiogenesis in vitro via CXCL8 and CCL2 in retinal 
      pigment epithelium.
PG  - 4627-4632
LID - 10.3892/mmr.2018.8460 [doi]
AB  - Interleukin-17 (IL-17) is a major pro-inflammatory cytokine involved in choroidal 
      endothelial cell (CEC) angiogenesis. Proteins expressed by the retinal pigment 
      epithelium (RPE) may contribute to CEC angiogenesis. The ability of IL‑17 to 
      promote proliferation, migration and capillary‑like structure formation in CECs 
      was investigated by stimulating the RPE in vitro. CECs were cultured in a 
      conditioned medium (CM) with IL‑17 (IL‑17‑CM) or without IL‑17 (CM) obtained from 
      the supernatant of an ARPE‑19 cell line. The pro‑angiogenic role of IL‑17‑CM on 
      CECs was investigated with water‑soluble tetrazolium 1 analysis, wound healing 
      and Matrigel matrix tube formation assays. The expression level of vascular 
      endothelial growth factor was detected by enzyme‑linked immunosorbent assay in 
      RPE cells treated with or without IL‑17. Ras‑related C3botulinum toxin substrate 
      1 (Rac1) and Ras homolog gene family member A (RhoA) activities were analyzed by 
      pull‑down assays. IL‑17‑CM significantly enhanced tube formation and increased 
      the migration distance in CECs in comparison with CM. This effect was diminished 
      by neutralizing C‑C motif chemokine 2 (CCL2) and C‑X‑C motif chemokine ligand 8 
      (CXCL8) expression in IL‑17‑CM, with a concomitant downregulation of Rac1 and 
      RhoA activity in CECs. In conclusion, it was demonstrated that IL‑17 mediated the 
      expression of CCL2 and CXCL8 in RPE cells, resulting in increased migration and 
      tube formation in human CECs.
FAU - Chen, Ying
AU  - Chen Y
AD  - Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, 
      Chongqing 400016, P.R. China.
FAU - Zhong, Murui
AU  - Zhong M
AD  - Department of Ophthalmology, 452 Hospital of People's Liberation Army, Chengdu, 
      Sichuan 610065, P.R. China.
FAU - Yuan, Gangxiang
AU  - Yuan G
AD  - Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, 
      Chongqing 400016, P.R. China.
FAU - Peng, Hui
AU  - Peng H
AD  - Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, 
      Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180118
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-8)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Antibodies, Neutralizing/immunology
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/immunology/*metabolism
MH  - Choroid/cytology/metabolism
MH  - Culture Media, Conditioned/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-17/*pharmacology
MH  - Interleukin-8/immunology/*metabolism
MH  - Neovascularization, Physiologic/*drug effects
MH  - Retinal Pigment Epithelium/cytology/drug effects/metabolism
MH  - Vascular Endothelial Growth Factor A/analysis
MH  - rac1 GTP-Binding Protein/metabolism
MH  - rhoA GTP-Binding Protein/metabolism
OTO - NOTNLM
OT  - retinal pigment epithelium
OT  - choroidal endothelial cell
OT  - neovascularization
OT  - C‑C motif chemokine ligand 2
OT  - C‑X‑C motif chemokine ligand 8
EDAT- 2018/01/25 06:00
MHDA- 2018/08/15 06:00
CRDT- 2018/01/25 06:00
PHST- 2017/06/06 00:00 [received]
PHST- 2017/11/30 00:00 [accepted]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2018/01/25 06:00 [entrez]
AID - 10.3892/mmr.2018.8460 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Mar;17(3):4627-4632. doi: 10.3892/mmr.2018.8460. Epub 2018 Jan 
      18.