PMID- 2936392
OWN - NLM
STAT- MEDLINE
DCOM- 19860403
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 24
IP  - 26
DP  - 1985 Dec 17
TI  - Segmental flexibility of receptor-bound immunoglobulin E.
PG  - 7810-20
AB  - The segmental flexibility of mouse immunoglobulin E (IgE) bound to its 
      high-affinity receptor on membrane vesicles from rat basophilic leukemia cells 
      was compared to that of IgE in solution by measuring the steady-state anisotropy 
      as a function of temperature and viscosity. A monoclonal IgE was used to bind the 
      fluorescent probe N-[5-(dimethylamino)naphthalene-1-sulfonyl]-L-lysine (DNS-Lys) 
      rigidly and specifically in the antigen combining site at the tip of the Fab 
      region. The average rotational correlation time, phi, of 74-89 ns for the 
      receptor-bound IgE is only slightly longer than that for IgE in solution where 
      phi = 54 ns. Another mouse monoclonal IgE was covalently labeled in the Fab 
      region with N-(1-pyrenyl)maleimide. Anisotropy measurements with this derivative 
      yielded results that are very similar to those found with anti-DNS IgE and 
      DNS-Lys. These findings are strikingly different from that expected for a rigid 
      IgE bound to its receptor since in this case phi is likely to be very much 
      larger. Evidently, the segmental flexibility of IgE is not greatly altered upon 
      binding to its receptor.
FAU - Slattery, J
AU  - Slattery J
FAU - Holowka, D
AU  - Holowka D
FAU - Baird, B
AU  - Baird B
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI18610/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 0 (Solutions)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils
MH  - Cell Membrane/immunology
MH  - Fluorescence Polarization
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Experimental/immunology
MH  - Light
MH  - Mice
MH  - Molecular Conformation
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Receptors, IgE
MH  - Scattering, Radiation
MH  - Solutions
MH  - Spectrometry, Fluorescence
EDAT- 1985/12/17 00:00
MHDA- 1985/12/17 00:01
CRDT- 1985/12/17 00:00
PHST- 1985/12/17 00:00 [pubmed]
PHST- 1985/12/17 00:01 [medline]
PHST- 1985/12/17 00:00 [entrez]
AID - 10.1021/bi00347a046 [doi]
PST - ppublish
SO  - Biochemistry. 1985 Dec 17;24(26):7810-20. doi: 10.1021/bi00347a046.