PMID- 29364206
OWN - NLM
STAT- MEDLINE
DCOM- 20180619
LR  - 20190107
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 130
DP  - 2017 Dec 30
TI  - In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages.
LID - 10.3791/56322 [doi]
LID - 56322
AB  - Bone marrow-derived macrophages (BMDMs) are mature leukocytes that serve a 
      critical physiological role as professional phagocytes capable of clearing a 
      variety of particles. Normally, BMDMs are restricted from the central nervous 
      system (CNS), but following an injury, they can readily infiltrate. Once within 
      the injured CNS tissue, BMDMs are the primary cell type responsible for the 
      clearance of injury-derived cellular debris, including large quantities of lipid 
      rich myelin debris. The neuropathological ramifications of BMDM infiltration and 
      myelin debris phagocytosis within the CNS are complex and not well understood. 
      The protocols described here, allow for the direct in vitro study of BMDMs in the 
      context of CNS injury. We cover murine BMDM isolation and culture, myelin debris 
      preparation, and assays to assess BMDM myelin debris phagocytosis. These 
      techniques produce robust quantifiable results without the need for significant 
      specialized equipment or materials, yet can be easily customized to meet the 
      needs of researchers.
FAU - Rolfe, Alyssa J
AU  - Rolfe AJ
AD  - Department of Biomedical Sciences, College of Medicine, Florida State University.
FAU - Bosco, Dale B
AU  - Bosco DB
AD  - Department of Biomedical Sciences, College of Medicine, Florida State University.
FAU - Broussard, Erynn N
AU  - Broussard EN
AD  - Department of Biomedical Sciences, College of Medicine, Florida State University.
FAU - Ren, Yi
AU  - Ren Y
AD  - Department of Biomedical Sciences, College of Medicine, Florida State University; 
      yi.ren@med.fsu.edu.
LA  - eng
GR  - R01 GM072611/GM/NIGMS NIH HHS/United States
GR  - R01 GM100474/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20171230
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Macrophages/cytology/immunology/metabolism/*physiology
MH  - Mice
MH  - Myelin Sheath/immunology/metabolism/*physiology
MH  - Phagocytosis/*physiology
PMC - PMC5908409
EDAT- 2018/01/25 06:00
MHDA- 2018/06/21 06:00
PMCR- 2018/12/30
CRDT- 2018/01/25 06:00
PHST- 2018/01/25 06:00 [entrez]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2018/12/30 00:00 [pmc-release]
AID - 56322 [pii]
AID - 10.3791/56322 [doi]
PST - epublish
SO  - J Vis Exp. 2017 Dec 30;(130):56322. doi: 10.3791/56322.