PMID- 29364225
OWN - NLM
STAT- MEDLINE
DCOM- 20180621
LR  - 20200108
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 131
DP  - 2018 Jan 8
TI  - Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in 
      an Extracellular Matrix Extract (ECME)-based Three-dimensional System.
LID - 10.3791/56589 [doi]
LID - 56589
AB  - Embedded in the extracellular matrix (ECM), normal and neoplastic epithelial 
      cells intimately communicate with hematopoietic and non-hematopoietic cells, thus 
      greatly influencing normal tissue homeostasis and disease outcome. In breast 
      cancer, tumor-associated macrophages (TAMs) play a critical role in disease 
      progression, metastasis, and recurrence; therefore, understanding the mechanisms 
      of monocyte chemoattraction to the tumor microenvironment and their interactions 
      with tumor cells is important to control the disease. Here, we provide a detailed 
      description of a three-dimensional (3D) co-culture system of human breast cancer 
      (BrC) cells and human monocytes. BrC cells produced high basal levels of 
      regulated on-activation, normal T-cell expressed and secreted (RANTES), monocyte 
      chemoattractant protein-1 (MCP-1), and granulocyte-macrophage colony-stimulating 
      factor (G-CSF), while in co-culture with monocytes, pro-inflammatory cytokines 
      Interleukin (IL)-1 beta (IL-1beta) and IL-8 were enriched together with matrix 
      metalloproteinases (MMP)-1, MMP-2, and MMP-10. This tumor stroma microenvironment 
      promoted resistance to anoikis in MCF-10A 3D acini-like structures, 
      chemoattraction of monocytes, and invasion of aggressive BrC cells. The protocols 
      presented here provide an affordable alternative to study intra-tumor 
      communication and are an example of the great potential that in vitro 3D cell 
      systems provide to interrogate specific features of tumor biology related to 
      tumor aggression.
FAU - Espinoza-Sanchez, Nancy Adriana
AU  - Espinoza-Sanchez NA
AD  - Unidad de Investigacion en Virologia y Cancer, Hospital Infantil de Mexico 
      Federico Gomez; Programa de Doctorado en Ciencias Biomedicas, Facultad de 
      Medicina, Universidad Nacional Autonoma de Mexico.
FAU - Chimal-Ramirez, Gloria Karina
AU  - Chimal-Ramirez GK
AD  - Unidad de Investigacion en Virologia y Cancer, Hospital Infantil de Mexico 
      Federico Gomez.
FAU - Fuentes-Panana, Ezequiel Moises
AU  - Fuentes-Panana EM
AD  - Unidad de Investigacion en Virologia y Cancer, Hospital Infantil de Mexico 
      Federico Gomez; ezequiel.fuentes@alumni.bcm.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20180108
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Cell Communication/physiology
MH  - Cell Culture Techniques/*methods
MH  - Cell Line, Tumor
MH  - Extracellular Matrix/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - MCF-7 Cells
MH  - Monocytes/*cytology/metabolism
MH  - Tumor Microenvironment
PMC - PMC5908448
EDAT- 2018/01/25 06:00
MHDA- 2018/06/22 06:00
PMCR- 2020/01/08
CRDT- 2018/01/25 06:00
PHST- 2018/01/25 06:00 [entrez]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2018/06/22 06:00 [medline]
PHST- 2020/01/08 00:00 [pmc-release]
AID - 56589 [pii]
AID - 10.3791/56589 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Jan 8;(131):56589. doi: 10.3791/56589.