PMID- 29364278
OWN - NLM
STAT- MEDLINE
DCOM- 20180620
LR  - 20190107
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 131
DP  - 2018 Jan 6
TI  - Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and 
      Osteoclast Progenitors from Mice.
LID - 10.3791/56750 [doi]
LID - 56750
AB  - Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as 
      a representation of mesenchymal stem cells in vitro. They reside within the bone 
      marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to 
      red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell 
      populations from the bone marrow results in a very heterogeneous mix of various 
      cell populations, which can present challenges in experimental design and 
      confound data interpretation. Several isolation and culture techniques have been 
      developed in laboratories in order to obtain more or less homogeneous populations 
      of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs 
      and HSCs from mouse long bones: one method that yields a mixed population of 
      BMSCs and HSCs and one method that attempts to separate the two cell populations 
      based on adherence. Both methods provide cells suitable for osteogenic and 
      adipogenic differentiation experiments as well as functional assays.
FAU - Maridas, David E
AU  - Maridas DE
AD  - Maine Medical Center Research Institute; david.maridas@maine.edu.
FAU - Rendina-Ruedy, Elizabeth
AU  - Rendina-Ruedy E
AD  - Maine Medical Center Research Institute.
FAU - Le, Phuong T
AU  - Le PT
AD  - Maine Medical Center Research Institute.
FAU - Rosen, Clifford J
AU  - Rosen CJ
AD  - Maine Medical Center Research Institute.
LA  - eng
GR  - R01 AR061164/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20180106
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation/physiology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Osteoclasts/*cytology
PMC - PMC5908449
EDAT- 2018/01/25 06:00
MHDA- 2018/06/21 06:00
PMCR- 2019/01/06
CRDT- 2018/01/25 06:00
PHST- 2018/01/25 06:00 [entrez]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2019/01/06 00:00 [pmc-release]
AID - 56750 [pii]
AID - 10.3791/56750 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Jan 6;(131):56750. doi: 10.3791/56750.