PMID- 29364483
OWN - NLM
STAT- MEDLINE
DCOM- 20191009
LR  - 20231213
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 1
DP  - 2018 Jan
TI  - Regulation of DNA methylation and tumor suppression gene expression by miR-29b in 
      leukemia patients and related mechanisms.
PG  - 158-165
LID - 14113 [pii]
LID - 10.26355/eurrev_201801_14113 [doi]
AB  - OBJECTIVE: Leukemia is characterized as a kind of malignant clonal disease in 
      hematological stem cells. The study showed an abnormal level of DNA methylation 
      in leukemia cells, which further led to an abnormal expression of hematological 
      genes. This study investigated the role of miR-29b on the modulation of DNA 
      methylation and tumor suppressor gene expression in leukemia patients. PATIENTS 
      AND METHODS: A total of 21 leukemia patients were recruited for the collection of 
      monocytes. Methylation levels of promoter sequence of ESR1 and p15 genes were 
      analyzed by methylation assay kit combined with DHPLC. DNA microarray and qRT-PCR 
      were used to measure microRNA expressional profile, and bioinformatics plus 
      luciferase reporter assay confirmed target gene of miR-29b. After transfection 
      with miR-29b, promoter methylation levels of ESR1 and p15 gene were measured. 
      Protein expressions of DNMT1 DNA (cytosine-5)-methyltransferase 1 (DNMT1), DNA 
      (cytosine-5)-methyltransferase 3A (DNMT3A) and DNA (cytosine-5)-methyltransferase 
      3B (DNMT3B) were quantified. RESULTS: The methylation levels of the promoter 
      region of ESR1 and p15 genes in monocytes of leukemia patient were significantly 
      elevated (p < 0.05). DNA microarray and qRT-PCR confirmed the down-regulation of 
      miR-29b (p < 0.05). Luciferase reporter assay revealed DNMT1, DNMT3A and DNMT3B 
      as target genes of miR-29b. MiR-29b transfection inhibited the expressions of 
      DNMT3A and DNMT3B in Kasumi-1 cells (p < 0.05), and promoter methylation levels 
      of estrogen Receptor 1 (ESR1) and p15 gene were decreased (p < 0.05). 
      CONCLUSIONS: In leukemia cells, hyper- methylation existed in the promoter region 
      of tumor suppressor gene. The methylation was enhanced in gene DNMT1, DNMT3A and 
      DNMT3B via the reduction of miR-29b in leukemia tumor cells.
FAU - Chen, Y-L
AU  - Chen YL
AD  - Department of Clinical Laboratory, Huzhou Central Hospital, Huzhou, Zhejiang, 
      China. jiayindirr@21cn.com.
FAU - Zhang, Z-X
AU  - Zhang ZX
FAU - Shou, L-H
AU  - Shou LH
FAU - Di, J-Y
AU  - Di JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p15)
RN  - 0 (DNMT3A protein, human)
RN  - 0 (ESR1 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (MIRN29a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA Methyltransferase 3A)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Cyclin-Dependent Kinase Inhibitor p15/genetics
MH  - DNA (Cytosine-5-)-Methyltransferases/chemistry/genetics/metabolism
MH  - *DNA Methylation
MH  - DNA Methyltransferase 3A
MH  - Estrogen Receptor alpha/genetics
MH  - Female
MH  - Humans
MH  - Leukemia/genetics/*pathology
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Monocytes/cytology/metabolism
MH  - Promoter Regions, Genetic
MH  - DNA Methyltransferase 3B
EDAT- 2018/01/25 06:00
MHDA- 2019/10/11 06:00
CRDT- 2018/01/25 06:00
PHST- 2018/01/25 06:00 [entrez]
PHST- 2018/01/25 06:00 [pubmed]
PHST- 2019/10/11 06:00 [medline]
AID - 14113 [pii]
AID - 10.26355/eurrev_201801_14113 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Jan;22(1):158-165. doi: 
      10.26355/eurrev_201801_14113.