PMID- 29371232
OWN - NLM
STAT- MEDLINE
DCOM- 20190318
LR  - 20190709
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 217
IP  - 4
DP  - 2018 Apr 2
TI  - MARCH1 protects the lipid raft and tetraspanin web from MHCII proteotoxicity in 
      dendritic cells.
PG  - 1395-1410
LID - 10.1083/jcb.201611141 [doi]
AB  - Dendritic cells (DCs) produce major histocompatibility complex II (MHCII) in 
      large amounts to function as professional antigen presenting cells. 
      Paradoxically, DCs also ubiquitinate and degrade MHCII in a constitutive manner. 
      Mice deficient in the MHCII-ubiquitinating enzyme membrane-anchored RING-CH1, or 
      the ubiquitin-acceptor lysine of MHCII, exhibit a substantial reduction in the 
      number of regulatory T (Treg) cells, but the underlying mechanism was unclear. 
      Here we report that ubiquitin-dependent MHCII turnover is critical to maintain 
      homeostasis of lipid rafts and the tetraspanin web in DCs. Lack of MHCII 
      ubiquitination results in the accumulation of excessive quantities of MHCII in 
      the plasma membrane, and the resulting disruption to lipid rafts and the 
      tetraspanin web leads to significant impairment in the ability of DCs to engage 
      and activate thymocytes for Treg cell differentiation. Thus, ubiquitin-dependent 
      MHCII turnover represents a novel quality-control mechanism by which DCs maintain 
      homeostasis of membrane domains that support DC's Treg cell-selecting function.
CI  - (c) 2018 Oh et al.
FAU - Oh, Jaehak
AU  - Oh J
AUID- ORCID: 0000-0003-3079-5927
AD  - Department of Microbiology and Immunology, University of California, San 
      Francisco, San Francisco, CA.
AD  - Sandler Asthma Basic Research Center, University of California, San Francisco, 
      San Francisco, CA.
FAU - Perry, Justin S A
AU  - Perry JSA
AD  - Department of Pathology, University of California, San Francisco, San Francisco, 
      CA.
FAU - Pua, Heather
AU  - Pua H
AUID- ORCID: 0000-0002-9271-2608
AD  - Sandler Asthma Basic Research Center, University of California, San Francisco, 
      San Francisco, CA.
AD  - Department of Pathology, University of California, San Francisco, San Francisco, 
      CA.
FAU - Irgens-Moller, Nicole
AU  - Irgens-Moller N
AD  - Department of Microbiology and Immunology, University of California, San 
      Francisco, San Francisco, CA.
AD  - Sandler Asthma Basic Research Center, University of California, San Francisco, 
      San Francisco, CA.
FAU - Ishido, Satoshi
AU  - Ishido S
AD  - Department of Microbiology, Hyogo College of Medicine 1-1, Mukogawa-cho, 
      Nishinomiya, Japan.
FAU - Hsieh, Chyi-Song
AU  - Hsieh CS
AD  - Department of Internal Medicine, Division of Rheumatology, Washington University 
      School of Medicine, St. Louis, MO.
FAU - Shin, Jeoung-Sook
AU  - Shin JS
AUID- ORCID: 0000-0002-0711-8234
AD  - Department of Microbiology and Immunology, University of California, San 
      Francisco, San Francisco, CA jeoung-sook.shin@ucsf.edu.
AD  - Sandler Asthma Basic Research Center, University of California, San Francisco, 
      San Francisco, CA.
LA  - eng
GR  - R01 AI079187/AI/NIAID NIH HHS/United States
GR  - R01 GM105800/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180125
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Tetraspanins)
RN  - EC 2.3.2.27 (MARCH1 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - *Cell Communication
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*enzymology/immunology/pathology
MH  - Histocompatibility Antigens Class II/genetics/immunology/*metabolism
MH  - Homeostasis
MH  - Lymphocyte Activation
MH  - Membrane Microdomains/*enzymology/immunology/pathology
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Phenotype
MH  - Receptors, Antigen, T-Cell/genetics/immunology/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes, Regulatory/immunology/*metabolism/transplantation
MH  - Tetraspanins/immunology/*metabolism
MH  - Thymocytes/immunology/*metabolism
MH  - Ubiquitin-Protein Ligases/genetics/immunology/*metabolism
MH  - Ubiquitination
PMC - PMC5881489
EDAT- 2018/01/27 06:00
MHDA- 2019/03/19 06:00
PMCR- 2018/10/02
CRDT- 2018/01/27 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/10/25 00:00 [revised]
PHST- 2018/01/08 00:00 [accepted]
PHST- 2018/01/27 06:00 [pubmed]
PHST- 2019/03/19 06:00 [medline]
PHST- 2018/01/27 06:00 [entrez]
PHST- 2018/10/02 00:00 [pmc-release]
AID - jcb.201611141 [pii]
AID - 201611141 [pii]
AID - 10.1083/jcb.201611141 [doi]
PST - ppublish
SO  - J Cell Biol. 2018 Apr 2;217(4):1395-1410. doi: 10.1083/jcb.201611141. Epub 2018 
      Jan 25.