PMID- 29371537 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 2309-608X (Electronic) IS - 2309-608X (Linking) VI - 3 IP - 2 DP - 2017 Apr 26 TI - Antifungal Susceptibility Testing of Fusarium: A Practical Approach. LID - 10.3390/jof3020019 [doi] LID - 19 AB - In vitro susceptibility testing of Fusarium is becoming increasingly important because of frequency and diversity of infections and because resistance profiles are species-specific. Reference methods for antifungal susceptibility testing (AFST) are those of Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility (EUCAST), but breakpoints (BPs) have not yet been established. One of the problems is that phylogenetic distances between Fusarium species are much smaller than between species of, e.g., Candida. Epidemiological cutoff values (ECVs) for some Fusarium species have been determined in order to differentiate wild-type from non-wild-type isolates. In clinical routine, commercially available assays such as Etest, Sensititre or others provide essential agreement with reference methods. Our objective is to summarize antifungal susceptibility testing of Fusarium genus in the clinical laboratory: how to do it, when to do it, and how to interpret it. FAU - Al-Hatmi, Abdullah M S AU - Al-Hatmi AMS AD - Westerdijk Fungal Biodiversity Institute, PO Box 85167, 3508 AD Utrecht, The Netherlands. abdullaalhatmi@gmail.com. AD - Centre of Expertise in Mycology Radboud University Medical Centre, Canisius Wilhelmina Hospital, 6500HB Nijmegen, The Netherlands. abdullaalhatmi@gmail.com. AD - Ministry of Health, Directorate General of Health Services, PO Box 393, 100 Muscat, Oman. abdullaalhatmi@gmail.com. FAU - Curfs-Breuker, Ilse AU - Curfs-Breuker I AD - Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, 6500HB Nijmegen, The Netherlands. i.breuker@cwz.nl. FAU - de Hoog, G Sybren AU - de Hoog GS AD - Westerdijk Fungal Biodiversity Institute, PO Box 85167, 3508 AD Utrecht, The Netherlands. s.hoog@westerdijkinstitute.nl. AD - Basic Pathology Department, Federal University of Parana State, Curitiba, 81540-970 Parana, Brazil. s.hoog@westerdijkinstitute.nl. AD - Biological Sciences Department, Faculty of Science, King Abdulaziz University, PO Box 80203 Jeddah, Saudi Arabia. s.hoog@westerdijkinstitute.nl. FAU - Meis, Jacques F AU - Meis JF AD - Centre of Expertise in Mycology Radboud University Medical Centre, Canisius Wilhelmina Hospital, 6500HB Nijmegen, The Netherlands. jacques.meis@gmail.com. AD - Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, 6500HB Nijmegen, The Netherlands. jacques.meis@gmail.com. FAU - Verweij, Paul E AU - Verweij PE AD - Centre of Expertise in Mycology Radboud University Medical Centre, Canisius Wilhelmina Hospital, 6500HB Nijmegen, The Netherlands. Paul.Verweij@radboudumc.nl. AD - Department of Medical Microbiology, Radboud University, Nijmegen Medical Centre, 6500GS Nijmegen, The Netherlands. Paul.Verweij@radboudumc.nl. LA - eng PT - Journal Article PT - Review DEP - 20170426 PL - Switzerland TA - J Fungi (Basel) JT - Journal of fungi (Basel, Switzerland) JID - 101671827 PMC - PMC5715922 OTO - NOTNLM OT - CLSI OT - EUCAST OT - Etest OT - Fusarium OT - clinical laboratory OT - susceptibility test COIS- The authors declare no conflict of interest. EDAT- 2018/01/27 06:00 MHDA- 2018/01/27 06:01 PMCR- 2017/04/26 CRDT- 2018/01/27 06:00 PHST- 2017/03/12 00:00 [received] PHST- 2017/04/14 00:00 [revised] PHST- 2017/04/19 00:00 [accepted] PHST- 2018/01/27 06:00 [entrez] PHST- 2018/01/27 06:00 [pubmed] PHST- 2018/01/27 06:01 [medline] PHST- 2017/04/26 00:00 [pmc-release] AID - jof3020019 [pii] AID - jof-03-00019 [pii] AID - 10.3390/jof3020019 [doi] PST - epublish SO - J Fungi (Basel). 2017 Apr 26;3(2):19. doi: 10.3390/jof3020019.