PMID- 29372761
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181001
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 11
DP  - 2017 Nov 8
TI  - [Determination of patulin in fruits and jam by solid phase extraction-ultra 
      performance liquid chromatography].
PG  - 1160-1164
LID - 10.3724/SP.J.1123.2017.07005 [doi]
AB  - With magnesium silicate, silica gel, diatomite and calcium sulfate as raw 
      materials, a new solid phase extraction column was prepared through a series of 
      processes of grinding to ethanol homogenate, drying and packing into 
      polypropylene tube. The sample was hydrolyzed by pectinase, extracted by 
      acetonitrile and purified by solid phase extraction. The target compounds were 
      separated on a C18 column (100 mmx2.1 mm, 1.8 mum), using 0.8% (v/v) 
      tetrahydrofuran solution as mobile phase with a flow rate of 0.5 mL/min. The 
      detection wavelength was 276 nm. The effect of pectinase on extraction yield and 
      purification effect of solid-phase extraction column were investigated. The 
      optimum chromatographic conditions were selected. There was a good linear 
      relationship between the peak heights and the mass concentrations of patulin in 
      the range of 0.1 to 10 mg/L with the correlation coefficient (R(2)) of 1. The 
      limit of detection for this method was 10.22 mug/kg. The spiked recoveries of 
      samples were 86.58%-94.84% with the relative standard deviations (RSDs) of 
      1.45%-2.28%. The results indicated that the self-made solid phase extraction 
      column had a good purification efficiency, and the UPLC had a high separation 
      efficiency. The method is simple, accurate and of great significance for the 
      quality and safety control of fruit products.
FAU - Lu, Weichao
AU  - Lu W
AD  - Analytical and Testing Center of Qiqihar University, Qiqihar 161006, China.
FAU - Shen, Shuchang
AU  - Shen S
AD  - Analytical and Testing Center of Qiqihar University, Qiqihar 161006, China. 
      sscfxzx@163.com.
FAU - Wang, Chao
AU  - Wang C
AD  - Analytical and Testing Center of Qiqihar University, Qiqihar 161006, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 95X2BV4W8R (Patulin)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Food Analysis/*methods
MH  - Fruit/*chemistry
MH  - Patulin/*analysis
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - fruit products
OT  - patulin (PAT)
OT  - solid phase extraction (SPE)
OT  - ultra performance liquid chromatography (UPLC)
EDAT- 2018/01/27 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/01/27 06:00
PHST- 2018/01/27 06:00 [entrez]
PHST- 2018/01/27 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.07005 [doi]
PST - ppublish
SO  - Se Pu. 2017 Nov 8;35(11):1160-1164. doi: 10.3724/SP.J.1123.2017.07005.