PMID- 29372762
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20180126
LR  - 20180126
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 11
DP  - 2017 Nov 8
TI  - [Determination of iminodiacetic acid and glycine in dehydrogenation products of 
      diethanolamine by pre-column derivatization and high performance liquid 
      chromatography].
PG  - 1165-1170
LID - 10.3724/SP.J.1123.2017.08006 [doi]
AB  - An analytical method was developed for the determination of iminodiacetic acid 
      (IDA) and glycine (Gly), the dehydrogenation products of diethanolamine, by high 
      performance liquid chromatography (HPLC) coupled with pre-column derivatization 
      using p-toluenesulfonyl chloride (PTSC) as the derivatization reagent. IDA and 
      Gly reacted with PTSC in the alkaline environment (pH 11) under 45℃ for 15 min. 
      Then the derivatization products were analyzed by HPLC-MS. The separation was 
      carried out on a high performance liquid chromatograph equipped with an 
      ultraviolet detector. A VP-ODS column (200 mmx4.6 mm, 5 mum) was employed using 
      0.03 mol/L ammonium acetate (pH 5.5)-acetonitrile (87:13, v/v) as mobile phases 
      for isocratic elution at a flow rate of 1 mL/min and detection wavelength of 235 
      nm. The results showed good linearities for iminodiacetic acid of 900-2100 mg/L, 
      and for glycine of 20-100 mg/L, respectively. The linear correlation coefficients 
      (R(2)) were both greater than 0.999. The limits of detection (LODs) of IDA and 
      Gly were 0.0897 mg/L and 0.0262 mg/L and the recoveries were in the range of 
      98.7%-99.3% and 98.0%-99.5%, respectively. The relative standard deviations 
      (RSDs) of IDA and Gly were in the range of 0.89%-1.23% and 0.95%-1.11% (n=3). The 
      method has the characteristics of mild reaction conditions and high accuracy, and 
      is well suitable for the determination of IDA and Gly in industrial production.
FAU - Zhang, Lei
AU  - Zhang L
AD  - College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
FAU - Duan, Zhengkang
AU  - Duan Z
AD  - College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China. 
      dzk0607@163.com.
FAU - Zhu, Hongwen
AU  - Zhu H
AD  - College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
FAU - Yin, Ke
AU  - Yin K
AD  - College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
OTO - NOTNLM
OT  - glycine (Gly)
OT  - high performance liquid chromatography (HPLC)
OT  - iminodiacetic acid (IDA)
OT  - p-toluenesulfonyl chloride (PTSC)
OT  - pre-column derivatization
EDAT- 2018/01/27 06:00
MHDA- 2018/01/27 06:01
CRDT- 2018/01/27 06:00
PHST- 2018/01/27 06:00 [entrez]
PHST- 2018/01/27 06:00 [pubmed]
PHST- 2018/01/27 06:01 [medline]
AID - 10.3724/SP.J.1123.2017.08006 [doi]
PST - ppublish
SO  - Se Pu. 2017 Nov 8;35(11):1165-1170. doi: 10.3724/SP.J.1123.2017.08006.