PMID- 29372775
OWN - NLM
STAT- MEDLINE
DCOM- 20181004
LR  - 20181004
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 35
IP  - 12
DP  - 2017 Dec 8
TI  - [Determination of hydroxyproline in liver tissue by hydrophilic interaction 
      chromatography-quadrupole/electrostatic field orbitrap high resolution mass 
      spectrometry].
PG  - 1251-1256
LID - 10.3724/SP.J.1123.2017.09020 [doi]
AB  - A method for the determination of hydroxyproline (Hyp) in liver tissue of mice by 
      hydrophilic interaction chromatography-quadrupole/electrostatic field orbitrap 
      high resolution mass spectrometry (HILIC-HRMS) was developed. The liver tissue 
      samples of normal mice and liver fibrosis mice induced by carbon tetrachloride 
      were hydrolyzed by concentrated hydrochloric acid. After filtrated and diluted by 
      solution, the diluent was separated on an Hypersil GOLD HILIC column (100 mmx2.1 
      mm, 3 mum). Water-acetonitrile (28:72, v/v)were used as the mobile phases with 
      isocratic elution. Finally, the target analytes were detected in positive model 
      by HRMS equipped with an electrospray ionization source. The linear range of 
      hydroxyproline was from 0.78 to 100.00 mug/L with the correlation coefficient 
      (R(2)) of 0.9983. The limit of quantification was 0.78 mug/L. By detecting the 
      spiked samples, the recoveries were in the range of 97.4%-100.9% with the 
      relative standard deviations (RSDs) between 1.4% and 2.0%. In addition, 
      comparison of the measurement results by this method and the chloramine T method 
      was proceeded. It was found that the linear correlation between the two methods 
      was very good, and the Pearson correlation coefficient was 0.927. And this method 
      had simpler operation procedure and higher accuracy than chloramine T method. 
      This method can be used for the quick determination of hydroxyproline in liver 
      tissue samples.
FAU - Liu, Wei
AU  - Liu W
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Qi, Shenglan
AU  - Qi S
AD  - Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese 
      Medicine, Shanghai 201203, China.
FAU - Xu, Ying
AU  - Xu Y
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Xiao, Zhun
AU  - Xiao Z
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - E-Institute of Shanghai Municipal Education Commission, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Fu, Yadong
AU  - Fu Y
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - E-Institute of Shanghai Municipal Education Commission, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Chen, Jiamei
AU  - Chen J
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
FAU - Yang, Tao
AU  - Yang T
AD  - Institute of Cardiovascular Disease, Shuguang Hospital Affiliated with Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China. 
      yangtao8579@163.com.
FAU - Liu, Ping
AU  - Liu P
AD  - Institute of Liver Disease, Shuguang Hospital Affiliated with Shanghai University 
      of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shanghai 
      University of Traditional Chinese Medicine, Shanghai 201203, China.
AD  - E-Institute of Shanghai Municipal Education Commission, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 201203, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - RMB44WO89X (Hydroxyproline)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Hydroxyproline/*analysis
MH  - Liver/*chemistry
MH  - Mass Spectrometry
MH  - Mice
MH  - Spectrum Analysis
MH  - Static Electricity
OTO - NOTNLM
OT  - hydrophilic interaction chromatography (HILIC)
OT  - hydroxyproline (Hyp)
OT  - liver fibrosis
OT  - liver tissue
OT  - quadrupole/orbitrap high resolution mass spectrometry (HRMS)
EDAT- 2018/01/27 06:00
MHDA- 2018/10/05 06:00
CRDT- 2018/01/27 06:00
PHST- 2018/01/27 06:00 [entrez]
PHST- 2018/01/27 06:00 [pubmed]
PHST- 2018/10/05 06:00 [medline]
AID - 10.3724/SP.J.1123.2017.09020 [doi]
PST - ppublish
SO  - Se Pu. 2017 Dec 8;35(12):1251-1256. doi: 10.3724/SP.J.1123.2017.09020.