PMID- 29373695
OWN - NLM
STAT- MEDLINE
DCOM- 20181015
LR  - 20191210
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 42
IP  - 5
DP  - 2018 Jun 1
TI  - Determination of Organophosphorous Pesticides in Blood Using Microextraction in 
      Packed Sorbent and Gas Chromatography-Tandem Mass Spectrometry.
PG  - 321-329
LID - 10.1093/jat/bky004 [doi]
AB  - The aim of our work was to develop a method for the determination of six 
      organophosphorous pesticides (Ops) (azynphos-ethyl (AZP), diazinon (DZN), 
      chlorpyrifos (CLP), chlorfenvinfos (CLF), parathion-ethyl (PRT) and quinalphos 
      (QLP)) in whole blood using microextraction by packed sorbent (MEPS) and analysis 
      by gas chromatography-tandem mass spectrometry (GC-MS/MS). The optimization of 
      the MEPS procedure was performed using a design of experiments (DOE) approach, 
      assessing different factors that significantly affected the extraction 
      efficiency. Ultimately, the number of sample strokes, wash volume, percentage of 
      2-propanol in the wash solvent and the number of elution strokes were 
      successfully optimized using a response surface methodology (RSM). The developed 
      and optimized method was fully validated according to international guidelines. 
      Linearity was established from 2.5 to 50 mug/mL for AZP and from 0.5 to 50 mug/mL 
      for the remaining compounds, with coefficients of determination (R2) higher than 
      0.99 in all cases. The lower limit of quantification were 2.5 mug/mL (AZP) and 0.5 
      mug/mL (remaining compounds). Recoveries ranged from 61% to 77%. Intra- and 
      inter-day precision and accuracy were considered adequate according to the 
      guidelines. This is the first method employing MEPS as a sample preparation 
      procedure for the analysis of these OPs in whole blood.
FAU - Santos, C
AU  - Santos C
AD  - Centro de Investigacao em Ciencias da Saude (CICS-UBI), Universidade da Beira 
      Interior, Av. Infante D. Henrique, 6201-556 Covilha, Portugal.
AD  - Laboratorio de Farmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, 
      EM506, 6201-556 Covilha, Portugal.
FAU - Oppolzer, D
AU  - Oppolzer D
AD  - Centro de Investigacao em Ciencias da Saude (CICS-UBI), Universidade da Beira 
      Interior, Av. Infante D. Henrique, 6201-556 Covilha, Portugal.
FAU - Goncalves, A
AU  - Goncalves A
AD  - Centro de Investigacao em Ciencias da Saude (CICS-UBI), Universidade da Beira 
      Interior, Av. Infante D. Henrique, 6201-556 Covilha, Portugal.
FAU - Barroso, M
AU  - Barroso M
AD  - Instituto Nacional de Medicina Legal e Ciencias Forenses-Delegacao do Sul, Rua 
      Manuel Bento de Sousa, 3, 1169-201 Lisboa, Portugal.
FAU - Gallardo, E
AU  - Gallardo E
AD  - Centro de Investigacao em Ciencias da Saude (CICS-UBI), Universidade da Beira 
      Interior, Av. Infante D. Henrique, 6201-556 Covilha, Portugal.
AD  - Laboratorio de Farmaco-Toxicologia, UBIMedical, Universidade da Beira Interior, 
      EM506, 6201-556 Covilha, Portugal.
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Organophosphates)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Organothiophosphorus Compounds)
RN  - 0 (Pesticides)
RN  - 0 (Solvents)
RN  - ND2M416302 (2-Propanol)
SB  - IM
MH  - 2-Propanol/chemistry
MH  - Analytic Sample Preparation Methods
MH  - Blood Banks
MH  - Calibration
MH  - Drug Stability
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Least-Squares Analysis
MH  - Limit of Detection
MH  - Molecular Structure
MH  - Organophosphates/*blood/chemistry/isolation & purification
MH  - Organophosphorus Compounds/blood/chemistry/isolation & purification
MH  - Organothiophosphorus Compounds/*blood/chemistry/isolation & purification
MH  - Pesticides/*blood/chemistry/isolation & purification
MH  - Reproducibility of Results
MH  - Solid Phase Microextraction
MH  - Solvents/chemistry
MH  - Tandem Mass Spectrometry
EDAT- 2018/01/27 06:00
MHDA- 2018/10/16 06:00
CRDT- 2018/01/27 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2018/01/27 06:00 [pubmed]
PHST- 2018/10/16 06:00 [medline]
PHST- 2018/01/27 06:00 [entrez]
AID - 4823130 [pii]
AID - 10.1093/jat/bky004 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2018 Jun 1;42(5):321-329. doi: 10.1093/jat/bky004.