PMID- 29373886 OWN - NLM STAT- MEDLINE DCOM- 20180619 LR - 20181202 IS - 1735-9694 (Electronic) IS - 0044-6025 (Linking) VI - 55 IP - 12 DP - 2017 Dec TI - The Neuroprotective Effects of Flaxseed Oil Supplementation on Functional Motor Recovery in a Model of Ischemic Brain Stroke: Upregulation of BDNF and GDNF. PG - 785-792 AB - Cerebral ischemic stroke is a common leading cause of disability. Flaxseed is a richest plant-based source of antioxidants. In this study, the effects of flaxseed oil (FSO) pretreatment on functional motor recovery and gene expression and protein content of neurotrophic factors in motor cortex area in rat model of brain ischemia/reperfusion (I/R) were assessed. Transient middle cerebral artery occlusion (tMCAo) in rats was used as model brain I/R. Rats (6 in each group) were randomly divided into four groups of Control (Co+normal saline [NS]), Sham (Sh+NS), tMCAo+NS and tMCAo+FSO. After three weeks of pretreatment with vehicle or FSO (0.2 ml~800 mg/kg body weight), the rats were operated in sham and ischemic groups. Ischemia was induced for 1 h and then reperfused. After 24 h of reperfusion, neurological examination was performed, and animals were sacrificed, and their brains were used for molecular and histopathological studies. FSO significantly improved the functional motor recovery compared with tMCAo+NS group (P<0.05). A significant reduction in brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) mRNAs and protein levels were observed in the tMCAo+NS group compared with Co+NS and Sh+NS group (P<0.05). A significant increase of BDNF and GDNF mRNAs and proteins was recorded in the tMCAo+FSO group compared with Co+NS, Sh+NS and tMCAO+NS groups (P<0.05). The results of the current study demonstrated that pretreatment with FSO had neuroprotective effects on motor cortex area following cerebral ischemic stroke by increasing the neurotrophic factors (BDNF, GDNF). FAU - Bagheri, Abolqasem AU - Bagheri A AD - Department of Anatomy, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran. FAU - Talei, Sahand AU - Talei S AD - School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FAU - Hassanzadeh, Negar AU - Hassanzadeh N AD - School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FAU - Mokhtari, Tahmineh AU - Mokhtari T AD - Department of Anatomy, School of Medicine, Research Center of Nervous System Stem Cells, Semnan University of Medical Sciences, Semnan, Iran. FAU - Akbari, Mohammad AU - Akbari M AD - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FAU - Malek, Fatemeh AU - Malek F AD - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FAU - Jameie, Seyed Behnamedin AU - Jameie SB AD - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Sadeghi, Yousef AU - Sadeghi Y AD - Department of Anatomy, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Hassanzadeh, Gholamreza AU - Hassanzadeh G AD - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. LA - eng PT - Journal Article PL - Iran TA - Acta Med Iran JT - Acta medica Iranica JID - 14540050R RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Neuroprotective Agents) RN - 8001-26-1 (Linseed Oil) SB - IM MH - Animals MH - Brain/pathology MH - Brain Ischemia/*drug therapy MH - Brain-Derived Neurotrophic Factor/genetics MH - Disease Models, Animal MH - Glial Cell Line-Derived Neurotrophic Factor/genetics MH - Infarction, Middle Cerebral Artery MH - Linseed Oil/*chemistry MH - Male MH - Neuroprotective Agents/*pharmacology MH - Rats MH - Rats, Wistar MH - Recovery of Function MH - Stroke/*drug therapy MH - Up-Regulation OTO - NOTNLM OT - BDNF OT - Brain stroke OT - Flaxseed oil OT - GDNF OT - Motor cortex EDAT- 2018/01/28 06:00 MHDA- 2018/06/21 06:00 CRDT- 2018/01/28 06:00 PHST- 2017/12/16 00:00 [accepted] PHST- 2018/01/28 06:00 [entrez] PHST- 2018/01/28 06:00 [pubmed] PHST- 2018/06/21 06:00 [medline] PST - ppublish SO - Acta Med Iran. 2017 Dec;55(12):785-792.