PMID- 29375488
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Identification and Characterization of 5' Untranslated Regions (5'UTRs) in 
      Zymomonas mobilis as Regulatory Biological Parts.
PG  - 2432
LID - 10.3389/fmicb.2017.02432 [doi]
LID - 2432
AB  - Regulatory RNA regions within a transcript, particularly in the 5' untranslated 
      region (5'UTR), have been shown in a variety of organisms to control the 
      expression levels of these mRNAs in response to various metabolites or 
      environmental conditions. Considering the unique tolerance of Zymomonas mobilis 
      to ethanol and the growing interest in engineering microbial strains with 
      enhanced tolerance to industrial inhibitors, we searched natural cis-regulatory 
      regions in this microorganism using transcriptomic data and bioinformatics 
      analysis. Potential regulatory 5'UTRs were identified and filtered based on 
      length, gene function, relative gene counts, and conservation in other organisms. 
      An in vivo fluorescence-based screening system was developed to confirm the 
      responsiveness of 36 5'UTR candidates to ethanol, acetate, and xylose stresses. 
      UTR_ZMO0347 (5'UTR of gene ZMO0347 encoding the RNA binding protein Hfq) was 
      found to down-regulate downstream gene expression under ethanol stress. Genomic 
      deletion of UTR_ZMO0347 led to a general decrease of hfq expression at the 
      transcript level and increased sensitivity for observed changes in Hfq expression 
      at the protein level. The role of UTR_ZMO0347 and other 5'UTRs gives us insight 
      into the regulatory network of Z. mobilis in response to stress and unlocks new 
      strategies for engineering robust industrial strains as well as for harvesting 
      novel responsive regulatory biological parts for controllable gene expression 
      platforms in this organism.
FAU - Cho, Seung Hee
AU  - Cho SH
AD  - Institute for Cellular and Molecular Biology, University of Texas at Austin, 
      Austin, TX, United States.
FAU - Haning, Katie
AU  - Haning K
AD  - Department of Chemical Engineering, Cockrell School of Engineering, University of 
      Texas at Austin, Austin, TX, United States.
FAU - Shen, Wei
AU  - Shen W
AD  - Hubei Key Laboratory of Industrial Biotechnology, Hubei Collaborative Innovation 
      Center for Green Transformation of Bio-resources, Environmental Microbial 
      Technology Center of Hubei Province, College of Life Sciences, Hubei University, 
      Wuhan, China.
FAU - Blome, Cameron
AU  - Blome C
AD  - Department of Chemical Engineering, Cockrell School of Engineering, University of 
      Texas at Austin, Austin, TX, United States.
FAU - Li, Runxia
AU  - Li R
AD  - Hubei Key Laboratory of Industrial Biotechnology, Hubei Collaborative Innovation 
      Center for Green Transformation of Bio-resources, Environmental Microbial 
      Technology Center of Hubei Province, College of Life Sciences, Hubei University, 
      Wuhan, China.
FAU - Yang, Shihui
AU  - Yang S
AD  - Hubei Key Laboratory of Industrial Biotechnology, Hubei Collaborative Innovation 
      Center for Green Transformation of Bio-resources, Environmental Microbial 
      Technology Center of Hubei Province, College of Life Sciences, Hubei University, 
      Wuhan, China.
FAU - Contreras, Lydia M
AU  - Contreras LM
AD  - Institute for Cellular and Molecular Biology, University of Texas at Austin, 
      Austin, TX, United States.
AD  - Department of Chemical Engineering, Cockrell School of Engineering, University of 
      Texas at Austin, Austin, TX, United States.
LA  - eng
PT  - Journal Article
DEP - 20171208
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5770649
OTO - NOTNLM
OT  - 5' UTR
OT  - 5' rapid amplification of cDNA ends (RACE)
OT  - Zymomonas mobilis
OT  - regulatory RNA
OT  - stress response
EDAT- 2018/01/30 06:00
MHDA- 2018/01/30 06:01
PMCR- 2017/12/08
CRDT- 2018/01/30 06:00
PHST- 2017/07/26 00:00 [received]
PHST- 2017/11/23 00:00 [accepted]
PHST- 2018/01/30 06:00 [entrez]
PHST- 2018/01/30 06:00 [pubmed]
PHST- 2018/01/30 06:01 [medline]
PHST- 2017/12/08 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.02432 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Dec 8;8:2432. doi: 10.3389/fmicb.2017.02432. eCollection 
      2017.