PMID- 29375720
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 1
DP  - 2018 Jan
TI  - Propofol promotes apoptosis and suppresses the HOTAIR-mediated mTOR/p70S6K 
      signaling pathway in melanoma cells.
PG  - 630-634
LID - 10.3892/ol.2017.7297 [doi]
AB  - Propofol is an intravenous anesthetic, which is widely used in clinical 
      anesthesia induction and maintenance and is critical in the sedation of patients. 
      However, the functions and mechanisms of propofol on apoptosis of melanoma cells 
      remain unclear. The present study investigated whether propofol promotes cell 
      apoptosis and suppresses the HOX transcript antisense RNA (HOTAIR)-mediated 
      mechanistic target of rapamycin (mTOR) pathway in melanoma cells. B16F10 cells 
      were cultured with different concentrations (0-10 microM) of propofol for 24 or 48 h. 
      Proliferation and apoptosis of B16F10 cells were detected using MTT assay and 
      flow cytometry. The pcDNA 3.1(-)-HOTAIR and pcDNA 3.1(-)-control plasmids were 
      transfected into B16F10 cells using Lipofectamine 2000. In the present study, 
      treatment with propofol significantly reduced viability, and induced apoptosis 
      and caspase-3 activity in melanoma cells. Propofol treatment significantly 
      inhibited HOTAIR expression and the expression of phosphorylated (p)-mTOR and p- 
      p70S6K protein in melanoma cells. Overexpression of HOTAIR significantly 
      increased viability of melanoma cells, and increased HOTAIR, p-mTOR and p-p70S6K 
      protein expression in melanoma cells. These results indicated that propofol 
      promotes apoptosis and suppresses the HOTAIR-mediated mTOR signaling pathway in 
      melanoma cells.
FAU - Shang, Zhiwei
AU  - Shang Z
AD  - Department of Dermatology, The First Affiliated Hospital of Henan University of 
      Science and Technology, Luoyang, Henan 471003, P.R. China.
FAU - Feng, Haixia
AU  - Feng H
AD  - Department of Dermatology, The First Affiliated Hospital of Henan University of 
      Science and Technology, Luoyang, Henan 471003, P.R. China.
FAU - Cui, Lisha
AU  - Cui L
AD  - Department of Dermatology, The First Affiliated Hospital of Henan University of 
      Science and Technology, Luoyang, Henan 471003, P.R. China.
FAU - Wang, Weiping
AU  - Wang W
AD  - Department of Dermatology, The First Affiliated Hospital of Henan University of 
      Science and Technology, Luoyang, Henan 471003, P.R. China.
FAU - Fu, Hongwei
AU  - Fu H
AD  - Department of Dermatology, The First Affiliated Hospital of Henan University of 
      Science and Technology, Luoyang, Henan 471003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171031
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5766068
OTO - NOTNLM
OT  - HOX transcript antisense RNA
OT  - mechanistic target of rapamycin
OT  - melanoma
OT  - propofol
EDAT- 2018/01/30 06:00
MHDA- 2018/01/30 06:01
PMCR- 2017/10/31
CRDT- 2018/01/30 06:00
PHST- 2015/12/07 00:00 [received]
PHST- 2017/06/29 00:00 [accepted]
PHST- 2018/01/30 06:00 [entrez]
PHST- 2018/01/30 06:00 [pubmed]
PHST- 2018/01/30 06:01 [medline]
PHST- 2017/10/31 00:00 [pmc-release]
AID - OL-0-0-7297 [pii]
AID - 10.3892/ol.2017.7297 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Jan;15(1):630-634. doi: 10.3892/ol.2017.7297. Epub 2017 Oct 31.