PMID- 29375985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 11
IP  - 1
DP  - 2018
TI  - EDIL3 depletion suppress epithelial-mesenchymal transition of lens epithelial 
      cells via transforming growth factor beta pathway.
PG  - 18-24
LID - 10.18240/ijo.2018.01.04 [doi]
AB  - AIM: To study the effect of discoidin I-like domaincontaining protein 3 (EDIL3) 
      depletion on the proliferation and epithelial-mesenchymal transition (EMT) in 
      human lens epithelial cells (LECs). METHODS: RNA interference was used to inhibit 
      the expression of EDIL3 in human LECs in vitro. The morphology of cells was 
      observed using an inverted microscope. Cell proliferation was assessed using EdU 
      kit. Cell migration was investigated using Transwell chamber and EMT of LECs was 
      assessed using confocal microscope and Western blotting. The transforming growth 
      factor beta (TGFbeta) pathway was investigated using Western blotting. RESULTS: The 
      data showed that silencing EDIL3 expression changed LECs morphology and 
      suppressed LECs proliferation (P<0.05) and migration (P<0.01). Furthermore, the 
      result of Western blotting showed that EDIL3 depletion reduced the expression of 
      alpha-smooth muscle actin (alpha-SMA) (P<0.001) and vimentin (P<0.01), while increased 
      the expression of E-cadherin (P<0.001). EDIL3 depletion could suppress the 
      phosphorylation of Smad2 (P<0.01) and Smad3 (P<0.01) and the activation of 
      exracellular signal regulated kinase (ERK) (P<0.05). CONCLUSION: The findings 
      indicate that EDIL3 might participate in the proliferation and EMT in LECs via 
      TGFbeta pathway and may be a potential therapeutic target for the treatment of 
      posterior capsule opacification.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Wei, You-Heng
AU  - Wei YH
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Sciences, Fudan University, Shanghai 200438, China.
FAU - Zhao, Chun-Yan
AU  - Zhao CY
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Song, Hong-Yuan
AU  - Song HY
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Shen, Ni
AU  - Shen N
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Cui, Xiao
AU  - Cui X
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Gao, Xin
AU  - Gao X
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Qi, Zhong-Tian
AU  - Qi ZT
AD  - Department of Microbiology, Second Military Medical University, Shanghai 200433, 
      China.
FAU - Zhong, Ming
AU  - Zhong M
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Shen, Wei
AU  - Shen W
AD  - Department of Ophthalmology, Changhai Hospital, Second Military Medical 
      University, Shanghai 200433, China.
LA  - eng
PT  - Journal Article
DEP - 20180118
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC5767652
OTO - NOTNLM
OT  - discoidin I-like domain-containing protein 3
OT  - epithelial-mesenchymal transition
OT  - human lens epithelial cells
OT  - transforming growth factor beta
EDAT- 2018/01/30 06:00
MHDA- 2018/01/30 06:01
PMCR- 2018/01/18
CRDT- 2018/01/30 06:00
PHST- 2017/06/28 00:00 [received]
PHST- 2017/10/19 00:00 [accepted]
PHST- 2018/01/30 06:00 [entrez]
PHST- 2018/01/30 06:00 [pubmed]
PHST- 2018/01/30 06:01 [medline]
PHST- 2018/01/18 00:00 [pmc-release]
AID - ijo-11-01-018 [pii]
AID - 10.18240/ijo.2018.01.04 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2018 Jan 18;11(1):18-24. doi: 10.18240/ijo.2018.01.04. 
      eCollection 2018.