PMID- 29377634
OWN - NLM
STAT- MEDLINE
DCOM- 20190816
LR  - 20190816
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 18
IP  - 12
DP  - 2018 Jun
TI  - In Immunopeptidomics We Need a Sniper Instead of a Shotgun.
PG  - e1700464
LID - 10.1002/pmic.201700464 [doi]
AB  - Immunopeptidomics employs the use of mass spectrometry to identify and quantify 
      peptides presented on the surface of cells by major histocompatibility complex 
      (MHC; human leukocyte antigen [HLA], in humans) molecules, an essential component 
      of adaptive immunity. Currently, immunopeptidomics follows the same or similar 
      workflows as the more established field of shotgun proteomics, yet inherent 
      differences between these two fields create significant drawbacks for the former. 
      In this viewpoint, we would like to highlight such technical issues and provide 
      suggestions for novel workflows that would increase peptide sequencing coverage, 
      depth, and confidence, collectively enhancing the capabilities of the field of 
      immunopeptidomics.
CI  - (c) 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Faridi, Pouya
AU  - Faridi P
AD  - Infection and Immunity Program, Biomedicine Discovery Institute and Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 
      Australia.
FAU - Purcell, Anthony W
AU  - Purcell AW
AUID- ORCID: 0000-0003-0532-8331
AD  - Infection and Immunity Program, Biomedicine Discovery Institute and Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 
      Australia.
FAU - Croft, Nathan Paul
AU  - Croft NP
AUID- ORCID: 0000-0002-2128-5127
AD  - Infection and Immunity Program, Biomedicine Discovery Institute and Department of 
      Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180307
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptide Fragments)
MH  - Histocompatibility Antigens Class I/analysis/immunology/*metabolism
MH  - Histocompatibility Antigens Class II/analysis/immunology/*metabolism
MH  - Humans
MH  - Peptide Fragments/analysis/immunology/*metabolism
MH  - Proteomics/*methods
MH  - Workflow
OTO - NOTNLM
OT  - fragmentation
OT  - immunopeptidomics
OT  - peptide identification
OT  - sequencing
EDAT- 2018/01/30 06:00
MHDA- 2019/08/17 06:00
CRDT- 2018/01/30 06:00
PHST- 2017/12/18 00:00 [received]
PHST- 2018/01/10 00:00 [revised]
PHST- 2018/01/30 06:00 [pubmed]
PHST- 2019/08/17 06:00 [medline]
PHST- 2018/01/30 06:00 [entrez]
AID - 10.1002/pmic.201700464 [doi]
PST - ppublish
SO  - Proteomics. 2018 Jun;18(12):e1700464. doi: 10.1002/pmic.201700464. Epub 2018 Mar 
      7.