PMID- 29380946
OWN - NLM
STAT- MEDLINE
DCOM- 20181029
LR  - 20181029
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 33
IP  - 4
DP  - 2018 Jun
TI  - A fluorescent aptasensor for analysis of adenosine triphosphate based on 
      aptamer-magnetic nanoparticles and its single-stranded complementary DNA labeled 
      carbon dots.
PG  - 640-646
LID - 10.1002/bio.3457 [doi]
AB  - A new fluorimetric aptasensor was designed for the determination of adenosine 
      triphosphate (ATP) based on magnetic nanoparticles (MNPs) and carbon dots (CDs). 
      In this analytical strategy, an ATP aptamer was conjugated on MNPs and a 
      complementary strand of the aptamer (CS) was labeled with CDs. The aptamer and 
      its CS were hybridized to form a double helical structure. The hybridized 
      aptamers could be used for the specific recognition of ATP in a biological 
      complex matrix using a strong magnetic field to remove the interfering effect. In 
      the absence of ATP, no CDs-CS could be released into the solution and this 
      resulted in a weak fluorescence signal. In the presence of ATP, the target binds 
      to its aptamer and causes the dissociation of the double helical structure and 
      liberation of the CS, such that a strong fluorescence signal was generated. The 
      increased fluorescence signal was proportional to ATP concentration. The limit of 
      detection was estimated to be 1.0 pmol L(-1) with a dynamic range of 3.0 pmol 
      L(-1) to 5.0 nmol L(-1) . The specific aptasensor was applied to detect ATP in 
      human serum samples with satisfactory results. Moreover, molecular dynamic 
      simulation (MDS) studies were used to analyze interactions of the ATP molecule 
      with the aptamer.
CI  - Copyright (c) 2018 John Wiley & Sons, Ltd.
FAU - Saberi, Zeinab
AU  - Saberi Z
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, Islamic 
      Republic of Iran.
FAU - Rezaei, Behzad
AU  - Rezaei B
AUID- ORCID: 0000-0003-0055-0486
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, Islamic 
      Republic of Iran.
FAU - Khayamian, Taghi
AU  - Khayamian T
AD  - Department of Chemistry, Isfahan University of Technology, Isfahan, Islamic 
      Republic of Iran.
LA  - eng
PT  - Journal Article
DEP - 20180130
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Magnetite Nanoparticles)
RN  - 7440-44-0 (Carbon)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*blood
MH  - Aptamers, Nucleotide/*chemistry
MH  - Carbon/*chemistry
MH  - DNA, Single-Stranded/*chemistry
MH  - Fluorescence
MH  - Humans
MH  - Magnetite Nanoparticles/*chemistry
MH  - Models, Molecular
MH  - Particle Size
MH  - Quantum Dots/*chemistry
MH  - Surface Properties
OTO - NOTNLM
OT  - adenosine triphosphate
OT  - aptamer
OT  - carbon dots
OT  - complementary strand of aptamer
EDAT- 2018/01/31 06:00
MHDA- 2018/10/30 06:00
CRDT- 2018/01/31 06:00
PHST- 2017/08/26 00:00 [received]
PHST- 2017/10/16 00:00 [revised]
PHST- 2017/12/24 00:00 [accepted]
PHST- 2018/01/31 06:00 [pubmed]
PHST- 2018/10/30 06:00 [medline]
PHST- 2018/01/31 06:00 [entrez]
AID - 10.1002/bio.3457 [doi]
PST - ppublish
SO  - Luminescence. 2018 Jun;33(4):640-646. doi: 10.1002/bio.3457. Epub 2018 Jan 30.