PMID- 29382231
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20190624
IS  - 1557-7422 (Electronic)
IS  - 1043-0342 (Linking)
VI  - 29
IP  - 7
DP  - 2018 Jul
TI  - Overexpression of Hepatocyte Growth Factor mRNA Induced by Gene Transfer 
      Attenuates Neointimal Hyperplasia After Balloon Injury.
PG  - 816-827
LID - 10.1089/hum.2017.173 [doi]
AB  - Hepatic growth factor (HGF) has been widely used in studies on arterial 
      remodeling after injury, and results turn out to be inconsistent. The changes of 
      endogenous HGF expression after injury also remain controversial. This study 
      clarified the role of exogenous human HGF (hHGF) gene transfer in neointimal 
      hyperplasia and investigated the associated alterations of endogenous HGF and 
      c-Met expressions under endothelial denudation with or without hHGF gene transfer 
      using a balloon-injured rabbit aorta model. Sixty-one rabbits were randomly 
      divided into normal controls, endothelial injury, endothelial injury with hHGF, 
      or the control vector gene transfer groups. On weeks 1, 2, 4, and 8 after injury, 
      neointimal hyperplasia and endothelialization were evaluated by the ratio of 
      neointimal area to medial area (N/M ratio), CD31-positive staining, alpha-smooth 
      muscle actin, and endothelial nitric oxide synthase expressions using 
      histological analysis, immunohistochemistry staining, or real-time quantitative 
      reverse transcriptase polymerase chain reaction. Endogenous rabbit HGF (rHGF) and 
      c-Met expressions were detected with immunohistochemistry staining and 
      quantitative reverse transcriptase polymerase chain reaction. It was found that 
      expressions of endogeneous rHGF and c-Met in endothelial injury upregulated with 
      peak levels on week 2 or week 4 after injury (p < 0.01). On week 1 after hHGF 
      transfer, neointimal hyperplasia was significantly inhibited (p < 0.001), with 
      decreased alpha-smooth muscle actin expression (p < 0.05) and improved endothelial 
      cells regeneration and function (p < 0.01). More remarkable overexpression of 
      endogenous rHGF and c-Met mRNAs were detected, and lowered positive staining of 
      rHGF and c-Met was shown in the neointima (p < 0.05). These results demonstrated 
      hHGF gene transfer induced further overexpression of endogenous rHGF and c-Met 
      mRNAs but lowered immunoreactivities of rHGF and c-Met in the neointima, thus 
      leading to significant attenuation of neointimal hyperplasia.
FAU - He, Yu
AU  - He Y
AD  - 1 Department of Ultrasound, China-Japan Union Hospital of Jilin University , 
      Changchun, China.
FAU - Mei, Li
AU  - Mei L
AD  - 2 Department of Ultrasound, the First Hospital of Jilin University , Changchun, 
      China .
FAU - Jin, Ying
AU  - Jin Y
AD  - 3 Department of Surgery, the First Hospital of Jilin University , Changchun, 
      China .
FAU - Li, Xiao-Ping
AU  - Li XP
AD  - 1 Department of Ultrasound, China-Japan Union Hospital of Jilin University , 
      Changchun, China.
FAU - Jin, Chunxiang
AU  - Jin C
AD  - 1 Department of Ultrasound, China-Japan Union Hospital of Jilin University , 
      Changchun, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180314
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Actins)
RN  - 0 (HGF protein, human)
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Actins/genetics
MH  - Animals
MH  - Endothelial Cells/drug effects/metabolism
MH  - Gene Expression Regulation/genetics
MH  - *Genetic Therapy
MH  - Genetic Vectors/administration & dosage
MH  - Hepatocyte Growth Factor/*administration & dosage/genetics
MH  - Humans
MH  - Hyperplasia/genetics/pathology/*therapy
MH  - Neointima/genetics/pathology/*therapy
MH  - Proto-Oncogene Proteins c-met/*genetics
MH  - RNA, Messenger/genetics
MH  - Rabbits
OTO - NOTNLM
OT  - balloon injury
OT  - gene transfer
OT  - hepatocyte growth factor (HGF)
OT  - neointimal hyperplasia
OT  - overexpression
EDAT- 2018/02/01 06:00
MHDA- 2019/06/25 06:00
CRDT- 2018/02/01 06:00
PHST- 2018/02/01 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2018/02/01 06:00 [entrez]
AID - 10.1089/hum.2017.173 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2018 Jul;29(7):816-827. doi: 10.1089/hum.2017.173. Epub 2018 Mar 
      14.