PMID- 29382416
OWN - NLM
STAT- MEDLINE
DCOM- 20180809
LR  - 20181202
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 33
IP  - 12
DP  - 2017 Dec
TI  - [Caspase-1 inhibitor AC-YVAD-CMK blocks IL-1beta secretion of bone marrow-derived 
      macrophages induced by Acinetobacter baumannii].
PG  - 1594-1599
AB  - Objective To explore the effect of caspase-1 selective inhibitor AC-YVAD-CMK on 
      IL-1beta secretion of bone marrow-derived macrophages (BMDMs) induced by 
      Acinetobacter baumannii (A. baumannii). Methods Macrophages were separated from 
      C57BL/6 mice which were stimulated using different concentrations of A. 
      baumannii. The level of IL-1beta in the culture supernatant was detected by ELISA. 
      The expression of pro-IL-1beta mRNA and protein were detected using the real-time 
      quantitative PCR and Western blot analysis, respectively. The role of caspase-1 
      in the secretion of IL-1beta was tested by AC-YVAD-CMK treatment to block caspase-1. 
      Pneumonia models in C57BL/6 mice were prepared by A. baumannii inoculation. The 
      level of IL-1beta in bronchoalveolar lavage fluid (BALF) and the morphology of lung 
      were detected by ELISA or HE staining, respectively. Results IL-1beta level in the 
      culture supernatant was up-reregulated by A. baumannii stimulation in a 
      dose-dependent manner. The expression of pro-IL-1beta mRNA and protein were not 
      significantly changed with A. baumannii stimulation. Mature IL-1beta secretion was 
      blocked by AC-YVAD-CMK either in vitro or in vivo. The damage of lung induced by 
      A. baumannii infection in mice was ameliorated by AC-YVAD-CMK. Conclusion 
      AC-YVAD-CMK alleviates pulmonary pathological damage by reducing the 
      caspase-1-mediated IL-1beta secretion.
FAU - Zhang, Yimin
AU  - Zhang Y
AD  - Department of Pathogenic Biology and Examination, Shaanxi University of Chinese 
      Medicine, Xianyang 712046, China. *Corresponding author, E-mail: 
      zhangyimin26@163.com.
FAU - Zhou, Xuening
AU  - Zhou X
AD  - Department of Clinical Laboratory, First Affiliated Hospital, Shaanxi University 
      of Chinese Medicine, Xianyang 712000, China.
FAU - Zhang, Hongfang
AU  - Zhang H
AD  - Department of Pathogenic Biology and Examination, Shaanxi University of Chinese 
      Medicine, Xianyang 712046, China.
FAU - Huan, Cheng
AU  - Huan C
AD  - Department of Pathogenic Biology and Examination, Shaanxi University of Chinese 
      Medicine, Xianyang 712046, China.
FAU - Ye, Zhengrong
AU  - Ye Z
AD  - Department of Pathogenic Biology and Examination, Shaanxi University of Chinese 
      Medicine, Xianyang 712046, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Amino Acid Chloromethyl Ketones)
RN  - 0 (Caspase Inhibitors)
RN  - 0 (Interleukin-1beta)
RN  - 0 (N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Acinetobacter baumannii/*pathogenicity
MH  - Amino Acid Chloromethyl Ketones/*pharmacology
MH  - Animals
MH  - Caspase 1/*physiology
MH  - Caspase Inhibitors/*pharmacology
MH  - Interleukin-1beta/*metabolism
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
EDAT- 2018/02/01 06:00
MHDA- 2018/08/10 06:00
CRDT- 2018/02/01 06:00
PHST- 2018/02/01 06:00 [entrez]
PHST- 2018/02/01 06:00 [pubmed]
PHST- 2018/08/10 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Dec;33(12):1594-1599.