PMID- 29382532
OWN - NLM
STAT- MEDLINE
DCOM- 20181009
LR  - 20181009
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 827
DP  - 2018 May 15
TI  - Cordycepin rescues lidocaine-induced neurotoxicity in dorsal root ganglion by 
      interacting with inflammatory signaling pathway MMP3.
PG  - 88-93
LID - S0014-2999(18)30062-1 [pii]
LID - 10.1016/j.ejphar.2018.01.049 [doi]
AB  - Application of local anesthetic reagents, such as lidocaine (Lid), could cause 
      significant neurotoxicity in spinal cord dorsal root ganglia neurons (DRGNs). In 
      this study, we investigated the potential rescuing effect of cordycepin (CDC) in 
      an in vitro explant model of lidocaine-induced apoptosis in DRGNs. Explant of rat 
      neonatal DRGNs was prepared, and treated with Lid in vitro to induce neuronal 
      apoptosis. Prior to Lid treatment, DRGN explant was pre-incubated with various 
      concentrations of CDC to evaluate its rescuing effect on Lid-induced apoptosis. 
      We found that, in cultured DRGNs, Lid caused significant neuronal apoptosis 
      whereas pre-incubation of CDC rescued Lid-induced neurotoxicity. In addition, 
      gene and protein expressions of Caspase-9 and Matrix metalloproteinase 3 (MMP3), 
      a key component of inflammatory signal pathway, were both upregulated by Lid but 
      downregulated by CDC pre-incubation. We further overexpressed MMP3 in cultured 
      DRGNs. And discovered that forced MMP3 overexpression upregulated endogenous MMP3 
      and caspase-9, and reversed the rescuing effect of CDC on Lid-induced 
      neurotoxicity in DRGNs. Therefore, we concluded that CDC has protective effect on 
      local anesthesia induced spinal cord neurotoxicity, possibly through the inverse 
      regulation on inflammatory signaling pathway.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Kan, Hongli
AU  - Kan H
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Wang, Yang
AU  - Wang Y
AD  - Department of Interventional Therapy, Jilin Provincial People's Hospital, 
      Changchun 130021, China. Electronic address: ericwang22@aol.com.
FAU - Wang, Dongxin
AU  - Wang D
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Sun, Hong
AU  - Sun H
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Zhou, Shu
AU  - Zhou S
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Wang, Huaiwu
AU  - Wang H
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Guan, Jian
AU  - Guan J
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
FAU - Li, Ming
AU  - Li M
AD  - Department of Anesthesia, Cancer Hospital of Jilin Province, Changchun 130012, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180131
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Deoxyadenosines)
RN  - 98PI200987 (Lidocaine)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - GZ8VF4M2J8 (cordycepin)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Deoxyadenosines/*pharmacology
MH  - Down-Regulation/drug effects
MH  - Ganglia, Spinal/*pathology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Inflammation/pathology
MH  - Lidocaine/*toxicity
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Neurons/*drug effects/pathology
MH  - Rats
MH  - Signal Transduction/*drug effects
OTO - NOTNLM
OT  - Anesthesia
OT  - Cordycepin
OT  - Dorsal root ganglion neuron
OT  - Lidocaine
OT  - MMP3
OT  - Spinal cord
EDAT- 2018/02/01 06:00
MHDA- 2018/10/10 06:00
CRDT- 2018/02/01 06:00
PHST- 2017/11/27 00:00 [received]
PHST- 2018/01/23 00:00 [revised]
PHST- 2018/01/26 00:00 [accepted]
PHST- 2018/02/01 06:00 [pubmed]
PHST- 2018/10/10 06:00 [medline]
PHST- 2018/02/01 06:00 [entrez]
AID - S0014-2999(18)30062-1 [pii]
AID - 10.1016/j.ejphar.2018.01.049 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2018 May 15;827:88-93. doi: 10.1016/j.ejphar.2018.01.049. Epub 
      2018 Jan 31.