PMID- 29388006
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181113
IS  - 1573-4986 (Electronic)
IS  - 0282-0080 (Linking)
VI  - 35
IP  - 2
DP  - 2018 Apr
TI  - Glycan recognition by human blood mononuclear cells with an emphasis on dendritic 
      cells.
PG  - 191-203
LID - 10.1007/s10719-017-9811-6 [doi]
AB  - Dendritic cells (DCs) play crucial roles in innate and adaptive immune response, 
      for which reason targeting antigen to these cells is an important strategy for 
      improvement of vaccine development. To this end, we explored recognition of DCs 
      lectins by glycans. For selection of the glycan "vector", a library of 229 
      fluorescent glycoprobes was employed to assess interaction with the 
      CD14(low/-)CD16(+)CD83(+) blood mononuclear cell population containing the DCs 
      known for their importance in antigen presentation to T-lymphocytes. It was found 
      that: 1) the glycan-binding profiles of this CD14(low/-)CD16(+)CD83(+) 
      subpopulation were similar but not identical to DCs of monocyte origin (moDCs); 
      2) the highest percentage of probe-positive cells in this CD14 
      (low/-)CD16(+)CD83(+) subpopulation was observed for GalNAcalpha1-2Galbeta (A(di)), 
      (Neu5Acalpha)(3) and three mannose-reach glycans; 3) subpopulation of 
      CD14(low/-)CD16(+) cells preferentially bound 4'-O-Su-LacdiNAc. Considering the 
      published data on specificity of DCs binding, the glycans showing particular 
      selectivity for the CD14 (low/-)CD16(+)CD83(+) cells are likely interacting with 
      macrophage galactose binding lectin (MGL), siglec-7 and dectin-2. In contrast, 
      DC-SIGN is not apparently involved, even in case of mannose-rich glycans. Taking 
      into consideration potential in vivo competition between glycan "vectors" and 
      glycans within glycocalyx, attempting to target vaccine to DCs glycan-binding 
      receptors should focus on A(di) and (Neu5Acalpha)(3) as the most promising vectors.
FAU - Rapoport, Eugenia M
AU  - Rapoport EM
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Khaidukov, Sergey V
AU  - Khaidukov SV
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Gaponov, Andrey M
AU  - Gaponov AM
AD  - D. Rogachyov Federal Research Center of Pediatric Hematology, Oncology and 
      Immunology, 117997, Samora Machela str., 1, Moscow, Russia.
FAU - Pazynina, Galina V
AU  - Pazynina GV
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Tsygankova, Svetlana V
AU  - Tsygankova SV
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Ryzhov, Ivan M
AU  - Ryzhov IM
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Belyanchikov, Ivan M
AU  - Belyanchikov IM
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia.
FAU - Milona, Panagiota
AU  - Milona P
AD  - Institute of Virology and Immunology, Sensemattstrasse 293, CH-3147, 
      Mittelhausern, Switzerland.
FAU - Bovin, Nicolai V
AU  - Bovin NV
AD  - Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997, 
      Miklukho-Maklaya str., 16/10, Moscow, Russia. professorbovin@yandex.ru.
FAU - McCullough, Kenneth C
AU  - McCullough KC
AD  - Institute of Virology and Immunology, Sensemattstrasse 293, CH-3147, 
      Mittelhausern, Switzerland.
LA  - eng
GR  - 16-04-01084/Russian Foundation for Basic Research/International
GR  - 16-54-76023/Russian Foundation for Basic Research/International
GR  - 601738/EU FP7 Project UniVax (HEALTH-F3-2013-60173)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180131
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Lectins)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Dendritic Cells/*metabolism
MH  - Humans
MH  - Lectins/chemistry/*metabolism
MH  - Monocytes/*metabolism
MH  - Polysaccharides/*metabolism
MH  - Protein Binding
OTO - NOTNLM
OT  - DC-SIGN
OT  - Dendritic cells
OT  - Glycans
OT  - Lectins
OT  - MGL
OT  - MMR
OT  - Monocytes
EDAT- 2018/02/02 06:00
MHDA- 2018/11/06 06:00
CRDT- 2018/02/02 06:00
PHST- 2017/10/05 00:00 [received]
PHST- 2017/12/27 00:00 [accepted]
PHST- 2017/12/25 00:00 [revised]
PHST- 2018/02/02 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/02/02 06:00 [entrez]
AID - 10.1007/s10719-017-9811-6 [pii]
AID - 10.1007/s10719-017-9811-6 [doi]
PST - ppublish
SO  - Glycoconj J. 2018 Apr;35(2):191-203. doi: 10.1007/s10719-017-9811-6. Epub 2018 
      Jan 31.