PMID- 29389309
OWN - NLM
STAT- MEDLINE
DCOM- 20181127
LR  - 20181127
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 18
IP  - 2
DP  - 2018 May 20
TI  - Standardization and optimization of fluorescence in situ hybridization (FISH) for 
      HER-2 assessment in breast cancer: A single center experience.
PG  - 132-140
LID - 10.17305/bjbms.2018.2519 [doi]
AB  - Accurate assessment of human epidermal growth factor receptor 2 (HER-2) is 
      crucial in selecting patients for targeted therapy. Commonly used methods for 
      HER-2 testing are immunohistochemistry (IHC) and fluorescence in situ 
      hybridization (FISH). Here we presented the implementation, optimization and 
      standardization of two FISH protocols using breast cancer samples and assessed 
      the impact of pre-analytical and analytical factors on HER-2 testing. Formalin 
      fixed paraffin embedded (FFPE) tissue samples from 70 breast cancer patients were 
      tested for HER-2 using PathVysion HER-2 DNA Probe Kit and two different paraffin 
      pretreatment kits, Vysis/Abbott Paraffin Pretreatment Reagent Kit (40 samples) 
      and DAKO Histology FISH Accessory Kit (30 samples). The concordance between FISH 
      and IHC results was determined. Pre-analytical and analytical factors (i.e., 
      fixation, baking, digestion, and post-hybridization washing) affected the 
      efficiency and quality of hybridization. The overall hybridization success in our 
      study was 98.6% (69/70); the failure rate was 1.4%. The DAKO pretreatment kit was 
      more time-efficient and resulted in more uniform signals that were easier to 
      interpret, compared to the Vysis/Abbott kit. The overall concordance between IHC 
      and FISH was 84.06%, kappa coefficient 0.5976 (p < 0.0001). The greatest 
      discordance (82%) between IHC and FISH was observed in IHC 2+ group. A 
      standardized FISH protocol for HER-2 assessment, with high hybridization 
      efficiency, is necessary due to variability in tissue processing and individual 
      tissue characteristics. Differences in the pre-analytical and analytical steps 
      can affect the hybridization quality and efficiency. The use of DAKO pretreatment 
      kit is time-saving and cost-effective.
FAU - Bogdanovska-Todorovska, Magdalena
AU  - Bogdanovska-Todorovska M
AD  - Institute of Pathology, Faculty of Medicine, Ss. Cyril and Methodius University, 
      Skopje, Republic of Macedonia. magde__b981@yahoo.com.
FAU - Petrushevska, Gordana
AU  - Petrushevska G
FAU - Janevska, Vesna
AU  - Janevska V
FAU - Spasevska, Liljana
AU  - Spasevska L
FAU - Kostadinova-Kunovska, Slavica
AU  - Kostadinova-Kunovska S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20180520
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - *Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*instrumentation/*methods
MH  - Nucleic Acid Hybridization
MH  - Paraffin Embedding
MH  - Receptor, ErbB-2/*genetics
MH  - Reproducibility of Results
PMC - PMC5988532
EDAT- 2018/02/02 06:00
MHDA- 2018/11/28 06:00
PMCR- 2018/05/01
CRDT- 2018/02/02 06:00
PHST- 2017/09/30 00:00 [received]
PHST- 2017/11/14 00:00 [accepted]
PHST- 2017/11/13 00:00 [revised]
PHST- 2018/02/02 06:00 [pubmed]
PHST- 2018/11/28 06:00 [medline]
PHST- 2018/02/02 06:00 [entrez]
PHST- 2018/05/01 00:00 [pmc-release]
AID - BJBMS-18-132 [pii]
AID - 10.17305/bjbms.2018.2519 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2018 May 20;18(2):132-140. doi: 10.17305/bjbms.2018.2519.