PMID- 29391899
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 1
DP  - 2018 Jan
TI  - CoCl(2) increases the expression of hypoxic markers HIF-1alpha, VEGF and CXCR4 in 
      breast cancer MCF-7 cells.
PG  - 1119-1124
LID - 10.3892/ol.2017.7369 [doi]
AB  - The aim of the present study was to investigate the effect of a hypoxic 
      environment on the biological behavior of breast cancer MCF-7 cells, using 
      CoCl(2) to mimic the hypoxia model in breast cancer cells. Using 50, 100, 150 and 
      200 microM CoCl(2) as a hypoxic inducer, a hypoxic model was established in MCF-7 
      cells in vitro. MTT, reverse transcription-quantitative polymerase chain reaction 
      (RT-qPCR), terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) 
      and western blotting assays were performed to detect MCF-7 cell proliferation 
      under hypoxic conditions and the expression of the hypoxic markers 
      hypoxia-inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF) 
      and C-X-C motif chemokine receptor 4 (CXCR4) mRNA and that of the associated 
      proteins. The RT-qPCR results revealed that there were no obvious changes in the 
      expression of HIF-1alpha mRNA; however, the expression of CXCR4 and VEGF mRNA 
      increased significantly following treatment with different CoCl(2) concentrations 
      (P<0.05). The results of western blotting identified that CoCl(2) significantly 
      induced the expression of HIF-1alpha, CXCR4 and VEGF proteins (P<0.05). The MTT assay 
      revealed that different concentrations of CoCl(2) inhibited the proliferation of 
      MCF-7 cells. The TUNEL assay demonstrated that CoCl(2) was able to trigger 
      apoptosis of MCF-7 cells. Therefore, the results of the present study identified 
      that CoCl(2) is able to control MCF-7 cell proliferation and apoptosis, also 
      increasing the expression of HIF-1alpha, CXCR4 and VEGF. The present study may aid 
      the discovery of a novel method to prevent cell damage and decrease cell 
      proliferation in order to prevent the occurrence and development of breast 
      cancer.
FAU - Li, Qing
AU  - Li Q
AD  - Department of Breast and Thyroid Surgery, Shandong Provincial Qianfoshan 
      Hospital, Shandong University, Jinan, Shandong 250000, P.R. China.
FAU - Ma, Rong
AU  - Ma R
AD  - Department of Breast and Thyroid Surgery, Shandong Provincial Qianfoshan 
      Hospital, Shandong University, Jinan, Shandong 250000, P.R. China.
FAU - Zhang, Mei
AU  - Zhang M
AD  - Department of Breast and Thyroid Surgery, Shandong Provincial Qianfoshan 
      Hospital, Shandong University, Jinan, Shandong 250000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171108
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5769392
OTO - NOTNLM
OT  - C-X-C motif chemokine receptor 4
OT  - CoCl2
OT  - MCF-7 cell
OT  - breast cancer
OT  - cell proliferation
OT  - hypoxia model
OT  - hypoxia-inducible factor-1alpha
OT  - vascular endothelial growth factor
EDAT- 2018/02/03 06:00
MHDA- 2018/02/03 06:01
PMCR- 2017/11/08
CRDT- 2018/02/03 06:00
PHST- 2016/08/12 00:00 [received]
PHST- 2017/09/13 00:00 [accepted]
PHST- 2018/02/03 06:00 [entrez]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2018/02/03 06:01 [medline]
PHST- 2017/11/08 00:00 [pmc-release]
AID - OL-0-0-7369 [pii]
AID - 10.3892/ol.2017.7369 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 Jan;15(1):1119-1124. doi: 10.3892/ol.2017.7369. Epub 2017 Nov 8.