PMID- 29392672
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20231213
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1744
DP  - 2018
TI  - In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves 
      Using TUNEL Assay.
PG  - 267-282
LID - 10.1007/978-1-4939-7672-0_21 [doi]
AB  - Leaf senescence constitutes a highly regulated final phase of leaf development, 
      leading to cell death that is recognized as a type of programmed cell death 
      (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) 
      and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL 
      reaction in situ labels free 3'-OH DNA strand breaks (nicks), thus allowing 
      histological localization of nuclear DNA degradation during PCD. Here we describe 
      in situ labeling of PCD-specific nuclear DNA fragmentation on conventional 
      histological sections of senescing tobacco leaves. Incorporation of 
      fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables 
      in situ visualization of PCD at the single-cell level in the leaf mesophyll 
      tissues undergoing senescence.
FAU - Uzelac, Branka
AU  - Uzelac B
AD  - Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 
      Belgrade, Serbia. branka@ibiss.bg.ac.rs.
FAU - Janosevic, Dusica
AU  - Janosevic D
AD  - Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, 
      University of Belgrade, Belgrade, Serbia.
FAU - Budimir, Snezana
AU  - Budimir S
AD  - Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 
      Belgrade, Serbia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Aging/*genetics
MH  - Apoptosis/*genetics
MH  - *In Situ Nick-End Labeling
MH  - Organ Specificity
MH  - Plant Leaves/*genetics/metabolism
MH  - Plant Physiological Phenomena/genetics
MH  - Nicotiana/*genetics/metabolism
OTO - NOTNLM
OT  - DNA fragmentation
OT  - Histology
OT  - Leaf senescence
OT  - Nicotiana tabacum
OT  - Programmed cell death
OT  - TUNEL assay
EDAT- 2018/02/03 06:00
MHDA- 2019/01/11 06:00
CRDT- 2018/02/03 06:00
PHST- 2018/02/03 06:00 [entrez]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
AID - 10.1007/978-1-4939-7672-0_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1744:267-282. doi: 10.1007/978-1-4939-7672-0_21.