PMID- 29392677
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20190110
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1744
DP  - 2018
TI  - Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by 
      Scanning Electron Microscope.
PG  - 321-328
LID - 10.1007/978-1-4939-7672-0_26 [doi]
AB  - Scanning electron microscope (SEM) is a type of electron microscope which 
      produces detailed images of surface structures. It has been widely used in plants 
      and animals to study cellular structures. Here, we describe a detailed protocol 
      to prepare samples of floral abscission zones (AZs) for SEM, as well as further 
      image analysis. We show that it is a powerful tool to detect morphologic changes 
      at the cellular level during the course of abscission in wild-type plants and to 
      establish the details of phenotypic alteration in abscission mutants.
FAU - Shi, Chun-Lin
AU  - Shi CL
AD  - Department of Molecular Biosciences, University of Oslo, Oslo, Norway.
FAU - Butenko, Melinka A
AU  - Butenko MA
AD  - Department of Molecular Biosciences, University of Oslo, Oslo, Norway. 
      m.a.butenko@ibv.uio.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Arabidopsis/*cytology/*ultrastructure
MH  - Cell Division
MH  - Cell Separation
MH  - *Microscopy, Electron, Scanning/methods
MH  - *Phenotype
MH  - Plant Cells/*ultrastructure
OTO - NOTNLM
OT  - Abscission
OT  - Abscission zone
OT  - Cell separation
OT  - Critical point drying
OT  - Floral organ abscission
OT  - Morphology
OT  - Scanning electron microscope
EDAT- 2018/02/03 06:00
MHDA- 2019/01/11 06:00
CRDT- 2018/02/03 06:00
PHST- 2018/02/03 06:00 [entrez]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
AID - 10.1007/978-1-4939-7672-0_26 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1744:321-328. doi: 10.1007/978-1-4939-7672-0_26.