PMID- 29393384
OWN - NLM
STAT- MEDLINE
DCOM- 20180823
LR  - 20181202
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 41
IP  - 4
DP  - 2018 Apr
TI  - Carbon monoxide releasing molecule‑3 promotes the osteogenic differentiation of 
      rat bone marrow mesenchymal stem cells by releasing carbon monoxide.
PG  - 2297-2305
LID - 10.3892/ijmm.2018.3437 [doi]
AB  - Stem cell‑based therapies are promising strategies to stimulate bone 
      regeneration. Carbon monoxide releasing molecule‑3 (CORM‑3) exhibits multiple 
      regulatory effects in a number of cells by releasing carbon monoxide (CO). The 
      present study aimed to investigate the influence of CORM‑3 on the osteogenic 
      differentiation of rat bone marrow mesenchymal stem cells (BMSCs). BMSCs were 
      divided into five groups: A CORM‑3‑osteogenic group, in which cells were 
      pretreated with CORM‑3 and subjected to osteogenic differentiation induction 
      using osteogenic medium; an osteogenic group, in which cells were cultured in 
      osteogenic medium; a degassed CORM‑3‑osteogenic group, in which cells were 
      pretreated with degassed CORM‑3 and subjected to osteogenic differentiation 
      induction; a CORM‑3 group, in which cells were cultured in control medium 
      containing CORM‑3; and a control group, in which cells were cultured in control 
      medium alone. The osteo‑specific mRNA and protein expression of runt‑related 
      transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN) were 
      assessed using reverse transcription‑quantitative polymerase chain reaction and 
      western blot analysis. Alkaline phosphatase (ALP) activity was also examined and 
      mineralization was detected using alizarin red staining. Levels of Runx2, OCN and 
      OPN mRNA and protein in the CORM‑3‑osteogenic group were significantly increased 
      compared with the osteogenic group (P<0.05), with the exception of OCN protein 
      levels on day 3. The mRNA and protein expression of Runx2, OCN and OPN in the 
      degassed CORM‑3‑osteogenic and osteogenic groups were similar. In addition, the 
      mRNA and protein expression of Runx2, OCN and OPN in the CORM‑3 and control group 
      were similar. ALP activity in the CORM‑3‑osteogenic group was increased from 
      day 3 and remained significantly higher compared with all other groups on 
      days 3, 5 and 7 (P<0.05). Additionally, the results indicated that the optical 
      density value of alizarin red staining in the CORM‑3‑osteogenic group was 
      significantly increased compared with the other groups (P<0.05). Therefore, the 
      present study demonstrated that CORM‑3 may promote the osteogenic differentiation 
      of BMSCs by releasing CO.
FAU - Li, Jingyuan
AU  - Li J
AD  - School of Dentistry, Shandong Provincial Key Laboratory of Oral Tissue 
      Regeneration, Shandong University, Jinan, Shandong 250012, P.R. China.
FAU - Song, Ling
AU  - Song L
AD  - Department of Stomatology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, 
      P.R. China.
FAU - Hou, Meng
AU  - Hou M
AD  - School of Stomatology, Medical College of Jining, Jining, Shandong 272000, P.R. 
      China.
FAU - Wang, Ping
AU  - Wang P
AD  - School of Dentistry, Shandong Provincial Key Laboratory of Oral Tissue 
      Regeneration, Shandong University, Jinan, Shandong 250012, P.R. China.
FAU - Wei, Lingling
AU  - Wei L
AD  - School of Dentistry, Shandong Provincial Key Laboratory of Oral Tissue 
      Regeneration, Shandong University, Jinan, Shandong 250012, P.R. China.
FAU - Song, Hui
AU  - Song H
AD  - School of Dentistry, Shandong Provincial Key Laboratory of Oral Tissue 
      Regeneration, Shandong University, Jinan, Shandong 250012, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180129
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Organometallic Compounds)
RN  - 0 (tricarbonylchloro(glycinato)ruthenium(II))
RN  - 7U1EE4V452 (Carbon Monoxide)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Carbon Monoxide/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Organometallic Compounds/*pharmacology
MH  - Osteogenesis/*drug effects
MH  - Rats
EDAT- 2018/02/03 06:00
MHDA- 2018/08/24 06:00
CRDT- 2018/02/03 06:00
PHST- 2017/08/06 00:00 [received]
PHST- 2018/01/09 00:00 [accepted]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2018/08/24 06:00 [medline]
PHST- 2018/02/03 06:00 [entrez]
AID - 10.3892/ijmm.2018.3437 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 Apr;41(4):2297-2305. doi: 10.3892/ijmm.2018.3437. Epub 2018 
      Jan 29.