PMID- 29393451
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20211105
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 4
DP  - 2018 Apr
TI  - MicroRNA‑195 is associated with regulating the pathophysiologic process of human 
      laryngeal squamous cell carcinoma.
PG  - 5283-5291
LID - 10.3892/mmr.2018.8523 [doi]
AB  - MicroRNAs (miRNAs) have been reported to be associated with the modulation of 
      tumor development, including alterations associated with the development of human 
      laryngeal squamous cell carcinoma (LSCC). The present study was designed to 
      investigate whether miRNA‑195 was associated with the pathophysiologic process of 
      human LSCC and to identify its potential roles and underlying molecular 
      mechanisms. To determine whether miRNA‑195 serves a role in LSCC, reverse 
      transcription‑quantitative polymerase chain reaction was used to detect miRNA‑195 
      expression in LSCC tissues. The tumor‑suppressive effect of miRNA‑195 was 
      determined by in vitro assays. Gain‑of‑function studies using miRNA‑195 mimics 
      were performed to investigate cell viability, migration and invasion, and 
      apoptosis in the AMC‑HN‑8 cell line. Western blotting was performed to reveal the 
      molecular mechanisms of miRNA‑195 and its downstream signaling pathways in the 
      LSCC AMC‑HN‑8 cell line. The present study demonstrated that miRNA‑195 is 
      downregulated in primary LSCC tumors. Upregulating miRNA‑195 in vitro suppressed 
      cell viability, migration and invasion in AMC‑HN‑8 cells. Overexpression of 
      miRNA‑195 alone in AMC‑HN‑8 cells was sufficient to induce cell apoptosis, as 
      identified by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. 
      Compared with the high expression of miRNA‑195 in AMC‑HN‑8 cells, the expression 
      levels of vascular endothelial growth factor receptor‑II protein and downstream 
      signaling pathway proteins, which were associated with cell viability, migration, 
      invasion and apoptosis, were markedly decreased compared with control or 
      miRNA‑195 negative control treatment group. Together, these data suggest the 
      therapeutic potential of miRNA‑195 in modulating cell growth, migration and 
      apoptosis during the pathophysiological progression of LSCC and that miRNA‑195 
      may serve as a potential therapeutic target in human LSCC.
FAU - Pang, Haifeng
AU  - Pang H
AD  - Department of Otolaryngology, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150001, P.R. China.
FAU - Xu, Xuemei
AU  - Xu X
AD  - Department of Otolaryngology, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150001, P.R. China.
FAU - Dai, Linlin
AU  - Dai L
AD  - Department of Otolaryngology, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150001, P.R. China.
FAU - Wang, Kun
AU  - Wang K
AD  - Department of Otolaryngology, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150001, P.R. China.
FAU - Yao, Xianyi
AU  - Yao X
AD  - Department of Otolaryngology, The Fourth Affiliated Hospital of Harbin Medical 
      University, Harbin, Heilongjiang 150001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180131
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (MIRN195 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.1 (KDR protein, human)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/genetics
MH  - Biomarkers
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/*pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Survival/genetics
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/*pathology
MH  - MAP Kinase Signaling System
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Models, Biological
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
OTO - NOTNLM
OT  - laryngeal squamous cell carcinoma
OT  - microRNA-195
OT  - vascular endothelial growth factor receptor 2
OT  - viability
OT  - migration
OT  - invasion
OT  - apoptosis
EDAT- 2018/02/03 06:00
MHDA- 2018/08/28 06:00
CRDT- 2018/02/03 06:00
PHST- 2016/01/13 00:00 [received]
PHST- 2017/12/20 00:00 [accepted]
PHST- 2018/02/03 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
PHST- 2018/02/03 06:00 [entrez]
AID - 10.3892/mmr.2018.8523 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Apr;17(4):5283-5291. doi: 10.3892/mmr.2018.8523. Epub 2018 Jan 
      31.