PMID- 29396547
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20240213
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Feb 2
TI  - Three-dimensional imaging of KNDy neurons in the mammalian brain using optical 
      tissue clearing and multiple-label immunocytochemistry.
PG  - 2242
LID - 10.1038/s41598-018-20563-2 [doi]
LID - 2242
AB  - Kisspeptin/Neurokinin B/Dynorphin (KNDy) neurons of the arcuate nucleus (ARC) 
      play a key role in the regulation of fertility. The ability to detect features of 
      KNDy neurons that are essential for fertility may require three-dimensional (3D) 
      imaging of the complete population. Recently developed protocols for optical 
      tissue clearing permits 3D imaging of neuronal populations in un-sectioned 
      brains. However, these techniques have largely been described in the mouse brain. 
      We report 3D imaging of the KNDy cell population in the whole rat brain and sheep 
      hypothalamus using immunolabelling and modification of a solvent-based clearing 
      protocol, iDISCO. This study expands the use of optical tissue clearing for 
      multiple mammalian models and provides versatile analysis of KNDy neurons across 
      species. Additionally, we detected a small population of previously unreported 
      kisspeptin neurons in the lateral region of the ovine mediobasal hypothalamus, 
      demonstrating the ability of this technique to detect novel features of the 
      kisspeptin system.
FAU - Moore, Aleisha M
AU  - Moore AM
AD  - Dept. of Neurobiology & Anatomical Sciences, University of Mississippi Medical 
      Center, Jackson, MS, USA.
FAU - Lucas, Kathryn A
AU  - Lucas KA
AD  - Dept. of Neurobiology & Anatomical Sciences, University of Mississippi Medical 
      Center, Jackson, MS, USA.
FAU - Goodman, Robert L
AU  - Goodman RL
AD  - Dept. of Physiology and Pharmacology, West Virginia University, Morgantown, West 
      Virginia, USA.
FAU - Coolen, Lique M
AU  - Coolen LM
AD  - Dept. of Neurobiology & Anatomical Sciences, University of Mississippi Medical 
      Center, Jackson, MS, USA. lcoolen@umc.edu.
AD  - Dept. of Physiology and Biophysics, University of Mississippi Medical Center, 
      Jackson, MS, USA. lcoolen@umc.edu.
FAU - Lehman, Michael N
AU  - Lehman MN
AD  - Dept. of Neurobiology & Anatomical Sciences, University of Mississippi Medical 
      Center, Jackson, MS, USA. mlehman@umc.edu.
LA  - eng
GR  - R01 HD039916/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20180202
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Kisspeptins)
RN  - 74913-18-1 (Dynorphins)
RN  - 86933-75-7 (Neurokinin B)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/cytology/*metabolism
MH  - Dynorphins/*metabolism
MH  - Female
MH  - Imaging, Three-Dimensional/methods
MH  - Immunohistochemistry/methods
MH  - Kisspeptins/*metabolism
MH  - Neurokinin B/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sheep
PMC - PMC5797235
COIS- The authors declare that they have no competing interests.
EDAT- 2018/02/06 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/02/02
CRDT- 2018/02/04 06:00
PHST- 2017/08/29 00:00 [received]
PHST- 2018/01/17 00:00 [accepted]
PHST- 2018/02/04 06:00 [entrez]
PHST- 2018/02/06 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/02/02 00:00 [pmc-release]
AID - 10.1038/s41598-018-20563-2 [pii]
AID - 20563 [pii]
AID - 10.1038/s41598-018-20563-2 [doi]
PST - epublish
SO  - Sci Rep. 2018 Feb 2;8(1):2242. doi: 10.1038/s41598-018-20563-2.