PMID- 29396644
OWN - NLM
STAT- MEDLINE
DCOM- 20190131
LR  - 20221207
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 22
IP  - 7
DP  - 2018 Sep
TI  - Expression and localization of CRAMP in rat tooth germ and during reparative 
      dentin formation.
PG  - 2559-2566
LID - 10.1007/s00784-018-2353-x [doi]
AB  - OBJECTIVES: Cathelicidin-related antimicrobial peptide (CRAMP) is an 
      antimicrobial peptide in mice and rats homologous to LL-37 in humans. In addition 
      to its antibacterial activity, CRAMP has various physiological functions by 
      binding to formyl peptide receptor 2 (FPR2). However, the role of these peptides 
      in teeth is unknown. Therefore, we investigated the role of CRAMP and FPR2 in 
      tooth development, reparative dentin formation, and defense response. MATERIAL 
      AND METHODS: First, we examined the localization of CRAMP and FPR2 during tooth 
      development by immunohistochemical analysis. Next, we investigated the 
      localization of CRAMP, FPR2, and CD68-positive macrophages by immunohistochemical 
      analysis during pulp inflammation and reparative dentin formation after cavity 
      preparation. Finally, we analyzed the effect of lipopolysaccharide (LPS) on the 
      expression of CRAMP and FPR2 in dental pulp cells by real-time reverse 
      transcription PCR. RESULTS: At the late bell stage in tooth development, CRAMP 
      was detected in odontoblasts, and FPR2 was observed in the sub-odontoblastic 
      layer. In mature teeth, CRAMP was not detected, but FPR2 continued to be 
      localized in the sub-odontoblastic layer. After cavity preparation, 
      CRAMP-positive cells and macrophages were found in dental pulp tissues below the 
      cavity at an early stage of repair. At subsequent stages of reparative dentin 
      formation, CRAMP was observed in odontoblast-like cells that contacted reparative 
      dentin. FPR2 immunoreactivity was also detected in odontoblast-like cells and 
      neighboring cells. LPS stimulated the expression of CRAMP mRNA in dental pulp 
      cells in vitro. CONCLUSIONS: Localization of CRAMP and its receptor FPR2-positive 
      cells were observed during physiological and reparative dentin formation. 
      CLINICAL RELEVANCE: CRAMP/LL-37 has a possibility that induce reparative dentin 
      formation.
FAU - Horibe, Kanji
AU  - Horibe K
AUID- ORCID: 0000-0002-8138-0046
AD  - Department of Oral Histology, Matsumoto Dental University, 1780 Hiro-oka, Gobara, 
      Shiojiri, Nagano, 399-0781, Japan. k_horibe@po.mdu.ac.jp.
FAU - Hosoya, Akihiro
AU  - Hosoya A
AD  - Department of Oral Histology, Matsumoto Dental University, 1780 Hiro-oka, Gobara, 
      Shiojiri, Nagano, 399-0781, Japan.
AD  - Department of Histology, School of Dentistry, Health Sciences University of 
      Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.
FAU - Hiraga, Toru
AU  - Hiraga T
AD  - Department of Oral Histology, Matsumoto Dental University, 1780 Hiro-oka, Gobara, 
      Shiojiri, Nagano, 399-0781, Japan.
FAU - Nakamura, Hiroaki
AU  - Nakamura H
AD  - Department of Oral Histology, Matsumoto Dental University, 1780 Hiro-oka, Gobara, 
      Shiojiri, Nagano, 399-0781, Japan.
LA  - eng
GR  - JP26893301/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20180202
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (CD68 protein, rat)
RN  - 0 (Receptors, Lipoxin)
RN  - 0 (lipoxin A(4) receptor, rat)
RN  - 0 (Cathelicidins)
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Antimicrobial Cationic Peptides/*metabolism
MH  - Cells, Cultured
MH  - Dental Cavity Preparation
MH  - Dentin, Secondary/*metabolism
MH  - Immunoenzyme Techniques
MH  - Odontoblasts/*metabolism
MH  - Odontogenesis/*physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptors, Lipoxin/*metabolism
MH  - Cathelicidins
OTO - NOTNLM
OT  - Antimicrobial peptide
OT  - Cavity preparation
OT  - Odontoblast
OT  - Reparative dentin
OT  - Tooth development
EDAT- 2018/02/06 06:00
MHDA- 2019/02/01 06:00
CRDT- 2018/02/04 06:00
PHST- 2017/09/07 00:00 [received]
PHST- 2018/01/22 00:00 [accepted]
PHST- 2018/02/06 06:00 [pubmed]
PHST- 2019/02/01 06:00 [medline]
PHST- 2018/02/04 06:00 [entrez]
AID - 10.1007/s00784-018-2353-x [pii]
AID - 10.1007/s00784-018-2353-x [doi]
PST - ppublish
SO  - Clin Oral Investig. 2018 Sep;22(7):2559-2566. doi: 10.1007/s00784-018-2353-x. 
      Epub 2018 Feb 2.