PMID- 29398481
OWN - NLM
STAT- MEDLINE
DCOM- 20190117
LR  - 20210109
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 10
IP  - 2
DP  - 2018 Feb 13
TI  - In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells.
PG  - 509-523
LID - S2213-6711(18)30027-4 [pii]
LID - 10.1016/j.stemcr.2018.01.001 [doi]
AB  - Due to differences across species, the mechanisms of cell fate decisions 
      determined in mice cannot be readily extrapolated to humans. In this study, we 
      developed a feeder- and xeno-free culture protocol that efficiently induced human 
      pluripotent stem cells (iPSCs) into PLZF+/GPR125+/CD90+ spermatogonium-like cells 
      (SLCs). These SLCs were enriched with key genes in germ cell development such as 
      MVH, DAZL, GFRalpha1, NANOS3, and DMRT1. In addition, a small fraction of SLCs went 
      through meiosis in vitro to develop into haploid cells. We further demonstrated 
      that this chemically defined induction protocol faithfully recapitulated the 
      features of compromised germ cell development of PSCs with NANOS3 deficiency or 
      iPSC lines established from patients with non-obstructive azoospermia. Taken 
      together, we established a powerful experimental platform to investigate human 
      germ cell development and pathology related to male infertility.
CI  - Copyright (c) 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Zhao, Yuncheng
AU  - Zhao Y
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Ye, Shicheng
AU  - Ye S
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Liang, Dongli
AU  - Liang D
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Wang, Pengxiang
AU  - Wang P
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Fu, Jing
AU  - Fu J
AD  - Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
FAU - Ma, Qing
AU  - Ma Q
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Kong, Ruijiao
AU  - Kong R
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Shi, Linghong
AU  - Shi L
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Gong, Xueping
AU  - Gong X
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Ding, Wubin
AU  - Ding W
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China.
FAU - Yang, Wenjing
AU  - Yang W
AD  - Systems Biology Center, National Heart, Lung, and Blood Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Zhu, Zijue
AU  - Zhu Z
AD  - Department of Andrology, Urologic Medical Center, Shanghai Key Lab of 
      Reproductive Medicine, Shanghai General Hospital, Shanghai Jiaotong University, 
      Shanghai 200080, China.
FAU - Chen, Huixing
AU  - Chen H
AD  - Department of Andrology, Urologic Medical Center, Shanghai Key Lab of 
      Reproductive Medicine, Shanghai General Hospital, Shanghai Jiaotong University, 
      Shanghai 200080, China.
FAU - Sun, Xiaoxi
AU  - Sun X
AD  - Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China.
FAU - Zhu, Jun
AU  - Zhu J
AD  - Systems Biology Center, National Heart, Lung, and Blood Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Li, Zheng
AU  - Li Z
AD  - Department of Andrology, Urologic Medical Center, Shanghai Key Lab of 
      Reproductive Medicine, Shanghai General Hospital, Shanghai Jiaotong University, 
      Shanghai 200080, China.
FAU - Wang, Yuan
AU  - Wang Y
AD  - Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences 
      and School of Life Sciences, East China Normal University, 500 Dongchuan Road, 
      Shanghai 200241, China; Department of Animal Science, Michigan State University, 
      Lansing, MI 48824, USA. Electronic address: ywang@bio.ecnu.edu.cn.
LA  - eng
GR  - R01 AA020404/AA/NIAAA NIH HHS/United States
GR  - ZIC HL006058/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180201
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (DAZL protein, human)
RN  - 0 (DMRT1 protein)
RN  - 0 (Gfra1 protein, mouse)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors)
RN  - 0 (NANOS3 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Azoospermia/*genetics/pathology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/genetics
MH  - Feeder Cells/cytology
MH  - Gene Expression Regulation, Developmental/genetics
MH  - Germ Cells/cytology/growth & development
MH  - Glial Cell Line-Derived Neurotrophic Factor Receptors/genetics
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology/pathology
MH  - Infertility, Male/*genetics/pathology
MH  - Male
MH  - Meiosis/genetics
MH  - Mice
MH  - Mouse Embryonic Stem Cells/cytology
MH  - RNA-Binding Proteins/genetics
MH  - Species Specificity
MH  - Spermatogonia/cytology/*growth & development/pathology
MH  - Transcription Factors/genetics
PMC - PMC5830957
OTO - NOTNLM
OT  - infertility
OT  - non-obstructive azoospermia
OT  - pluripotent stem cells
OT  - spermatogonia
EDAT- 2018/02/06 06:00
MHDA- 2019/01/18 06:00
PMCR- 2018/02/01
CRDT- 2018/02/06 06:00
PHST- 2017/04/04 00:00 [received]
PHST- 2017/12/28 00:00 [revised]
PHST- 2018/01/03 00:00 [accepted]
PHST- 2018/02/06 06:00 [pubmed]
PHST- 2019/01/18 06:00 [medline]
PHST- 2018/02/06 06:00 [entrez]
PHST- 2018/02/01 00:00 [pmc-release]
AID - S2213-6711(18)30027-4 [pii]
AID - 10.1016/j.stemcr.2018.01.001 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2018 Feb 13;10(2):509-523. doi: 10.1016/j.stemcr.2018.01.001. 
      Epub 2018 Feb 1.