PMID- 29398678
OWN - NLM
STAT- MEDLINE
DCOM- 20180411
LR  - 20201102
IS  - 1347-5223 (Electronic)
IS  - 0009-2363 (Linking)
VI  - 66
IP  - 4
DP  - 2018 Apr 1
TI  - Evaluation of Two Commercial Enzyme-Linked Immunosorbent Assay Kits for the 
      Detection of Human Circulating Metrnl.
PG  - 391-398
LID - 10.1248/cpb.c17-00846 [doi]
AB  - Metrnl is a newly discovered secreted protein with neurotrophic activity and 
      metabolic effect, while in earlier studies its circulating level in human was not 
      explored. We evaluated two commercial enzyme-linked immunosorbent assay kits 
      (DY7867-05, R&D Systems and SK00478-02, Aviscera Bioscience) for the detection of 
      human circulating Metrnl. The DY7867-05 kit showed superiority over the 
      SK00478-02 kit since it generated better curve fitting degree, smaller variation 
      among tests, higher inter-assay reproducibility and better specificity, and could 
      effectively detect human Metrnl in six types of blood samples. Subsequent 
      analysis was performed using the DY7867-05 kit. Sample storage conditions were 
      investigated. No gender difference in circulating Metrnl levels was found, while 
      people with newly diagnosed type 2 diabetes mellitus (T2DM) had significantly 
      lower Metrnl levels compared to the healthy controls.
FAU - Zheng, Si-Li
AU  - Zheng SL
AD  - Department of Pharmacology, Second Military Medical University.
FAU - Li, Zhi-Yong
AU  - Li ZY
AD  - Department of Pharmacology, Second Military Medical University.
FAU - Zhang, Zheng
AU  - Zhang Z
AD  - Department of Endocrinology, PLA 411 Hospital.
FAU - Wang, Dong-Sheng
AU  - Wang DS
AD  - Department of Pharmacology, Second Military Medical University.
FAU - Xu, Jian
AU  - Xu J
AD  - Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical 
      University.
FAU - Miao, Chao-Yu
AU  - Miao CY
AD  - Department of Pharmacology, Second Military Medical University.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20180202
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Adipokines)
RN  - 0 (Metrnl protein, human)
SB  - IM
MH  - Adipokines/*blood
MH  - Adult
MH  - Diabetes Mellitus, Type 2/*blood/diagnosis
MH  - Enzyme-Linked Immunosorbent Assay/*standards
MH  - Female
MH  - Humans
MH  - Male
OTO - NOTNLM
OT  - blood sample type
OT  - circulating Metrnl
OT  - enzyme-linked immunosorbent assay (ELISA)
OT  - gender difference
OT  - sample storage
OT  - type 2 diabetes mellitus (T2DM)
EDAT- 2018/02/06 06:00
MHDA- 2018/04/12 06:00
CRDT- 2018/02/06 06:00
PHST- 2018/02/06 06:00 [pubmed]
PHST- 2018/04/12 06:00 [medline]
PHST- 2018/02/06 06:00 [entrez]
AID - 10.1248/cpb.c17-00846 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 2018 Apr 1;66(4):391-398. doi: 10.1248/cpb.c17-00846. 
      Epub 2018 Feb 2.