PMID- 29402803
OWN - NLM
STAT- MEDLINE
DCOM- 20180503
LR  - 20200306
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 175
IP  - 3
DP  - 2018
TI  - Identification of Immunoglobulin E-Binding Proteins of the Xerophilic Fungus 
      Aspergillus penicillioides Crude Mycelial Mat Extract and Serological Reactivity 
      Assessment in Subjects with Different Allergen Reactivity Profiles.
PG  - 147-159
LID - 10.1159/000484898 [doi]
AB  - BACKGROUND: Aspergillus penicillioides is a very common indoor xerophilic fungus 
      and potential causative agent of respiratory conditions. Although people are 
      constantly exposed to A. penicillioides, no proteins with allergenic potential 
      have been described. Therefore, we aim to confirm allergic sensitization to A. 
      penicillioides through reactivity in serological assays and detect immunoglobulin 
      E (IgE)-binding proteins. METHODS: In an indirect ELISA, we compared the 
      serological reactivity to A. penicillioides between subjects with specific IgE 
      (sIgE) (group 1, n = 54) and no sIgE reactivity (group 2, n = 15) against 
      commercial allergens. Correlations and principal component analysis were 
      performed to identify associations between reactivity to commercial allergens and 
      A. penicillioides. IgE-binding proteins in A. penicillioides were visualized 
      using Western blotting (WB) in group 1. The IgE-binding proteins with the highest 
      reactivity were analyzed by mass spectrometry and confirmed by transcript 
      matching. RESULTS: There was no statistical significance (p = 0.1656) between the 
      study groups in serological reactivity. Correlations between reactivity to A. 
      penicillioides, dog epithelia, Aspergillus fumigatus, and Penicillium chrysogenum 
      were observed. WB experiments showed 6 IgE-binding proteins with molecular 
      weights ranging from 45 to 145 kDa. Proteins of 108, 83, and 56 kDa showed higher 
      reactivity. Mass spectrometry analysis of these 3 proteins led to the putative 
      identification of NADP-specific glutamate dehydrogenase and catalase B. This was 
      confirmed with transcriptome analysis. CONCLUSIONS: These results provide 
      evidence of the presence of potential allergenic components in A. penicillioides. 
      Further analysis of the putatively identified proteins should reveal their 
      allergenic potential.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Gonzalez De Leon, Joenice
AU  - Gonzalez De Leon J
AD  - Department of Microbiology and Medical Zoology, School of Medicine, University of 
      Puerto Rico, San Juan, PR, USA.
FAU - Gonzalez Mendez, Ricardo
AU  - Gonzalez Mendez R
FAU - Cadilla, Carmen L
AU  - Cadilla CL
FAU - Rivera-Mariani, Felix E
AU  - Rivera-Mariani FE
FAU - Bolanos-Rosero, Benjamin
AU  - Bolanos-Rosero B
LA  - eng
GR  - G12 MD007600/MD/NIMHD NIH HHS/United States
GR  - T36 GM095335/GM/NIGMS NIH HHS/United States
GR  - U54 MD007587/MD/NIMHD NIH HHS/United States
GR  - U54 MD007600/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180203
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Carrier Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Antibodies, Fungal/*immunology
MH  - Antigens, Fungal/*immunology
MH  - Aspergillus/*immunology
MH  - Blotting, Western
MH  - Carrier Proteins
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Mass Spectrometry
MH  - Pilot Projects
MH  - Principal Component Analysis
PMC - PMC5847473
MID - NIHMS930909
OTO - NOTNLM
OT  - <italic>Aspergillus penicillioides</italic>
OT  - Allergy
OT  - Immunoglobulin E-binding proteins
OT  - Serologic reactivity
OT  - Xerophilic fungi
COIS- Conflict of Interest: The authors have not conflict of interest to disclose.
EDAT- 2018/02/07 06:00
MHDA- 2018/05/04 06:00
PMCR- 2019/02/03
CRDT- 2018/02/07 06:00
PHST- 2017/06/02 00:00 [received]
PHST- 2017/10/31 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/05/04 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2019/02/03 00:00 [pmc-release]
AID - 000484898 [pii]
AID - 10.1159/000484898 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2018;175(3):147-159. doi: 10.1159/000484898. Epub 2018 
      Feb 3.