PMID- 29402845
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 45
IP  - 2
DP  - 2018
TI  - Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells 
      After Long-Term Exposure to Simulated Microgravity.
PG  - 429-445
LID - 10.1159/000486920 [doi]
AB  - BACKGROUND/AIMS: Cardiovascular complications are common in astronauts returning 
      from a prolonged spaceflight. These health problems might be driven by complex 
      modulations of gene expression and protein synthesis in endothelial cells (ECs). 
      Studies on the influence of microgravity on phenotype, growth pattern and 
      biological processes of ECs can help to understand these complications. METHODS: 
      We exposed ECs (EA.hy926) to a Random Positioning Machine (RPM). Proteins 
      associated with cell structure, angiogenesis and endothelial dysfunction were 
      investigated in distinct pools of multicellular spheroids (MCS), adherent cells 
      (AD) and tubular structures (TS) formed after a 35-day RPM-exposure. RESULTS: 
      Combining morphological and molecular approaches, we found AD, MCS and TS with 
      changes in the synthesis and release of proteins involved in three-dimensional 
      growth. Fibronectin and monocyte chemoattractant protein-1 (MCP-1) mRNAs and 
      protein contents were elevated along with an increased secretion of vascular 
      endothelial growth factor (VEGF), interleukin (IL)-6, IL-8, MCP-1, intercellular 
      adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), 
      neutrophil gelatinase-associated lipocalin (NGAL) and regulated on activation, 
      normal T cell expressed and secreted (RANTES) proteins in the culture supernatant 
      as determined by multianalyte profiling technology. Together they form a network 
      of interaction. CONCLUSIONS: These results show that a prolonged RPM-exposure of 
      ECs induced TS and MCS formation. The factors VEGF, NGAL, IL-6, IL-8, MCP-1, 
      VCAM-1, ICAM-1, fibronectin and RANTES seem to be affected when gravity is 
      omitted.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Dittrich, Anita
AU  - Dittrich A
AD  - Institute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark.
AD  - Medical Research Laboratory, Department of Clinical Medicine, Aarhus University, 
      Aarhus, Denmark.
FAU - Grimm, Daniela
AU  - Grimm D
AD  - Institute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark.
AD  - Gravitational Biology and Translational Regenerative Medicine, 
      Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.
AD  - Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University 
      Magdeburg, Magdeburg, Germany.
FAU - Sahana, Jayashree
AU  - Sahana J
AD  - Institute of Biomedicine, Pharmacology, Aarhus University, Aarhus, Denmark.
FAU - Bauer, Johann
AU  - Bauer J
AD  - Max-Planck-Institute of Biochemistry, Martinsried, Germany.
FAU - Kruger, Marcus
AU  - Kruger M
AD  - Gravitational Biology and Translational Regenerative Medicine, 
      Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.
AD  - Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University 
      Magdeburg, Magdeburg, Germany.
FAU - Infanger, Manfred
AU  - Infanger M
AD  - Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University 
      Magdeburg, Magdeburg, Germany.
FAU - Magnusson, Nils E
AU  - Magnusson NE
AD  - Medical Research Laboratory, Department of Clinical Medicine, Aarhus University, 
      Aarhus, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20180124
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipocalin-2)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - A549 Cells
MH  - Cell Adhesion
MH  - Cell Culture Techniques/instrumentation
MH  - Cell Fusion
MH  - Cell Line
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokine CCL5/analysis
MH  - Fibronectins/genetics/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-6/analysis
MH  - Interleukin-8/analysis
MH  - Lipocalin-2/analysis
MH  - *Neovascularization, Physiologic
MH  - Spheroids, Cellular/cytology/*metabolism
MH  - Up-Regulation
MH  - Vascular Cell Adhesion Molecule-1/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - *Weightlessness Simulation
OTO - NOTNLM
OT  - Angiogenesis
OT  - Endothelial cells
OT  - Endothelial dysfunction
OT  - Microgravity
OT  - Multicellular spheroids
OT  - Random Positioning Machine
OT  - Tube formation
EDAT- 2018/02/07 06:00
MHDA- 2018/03/27 06:00
CRDT- 2018/02/07 06:00
PHST- 2017/09/25 00:00 [received]
PHST- 2017/12/10 00:00 [accepted]
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
AID - 000486920 [pii]
AID - 10.1159/000486920 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;45(2):429-445. doi: 10.1159/000486920. Epub 2018 Jan 
      24.