PMID- 29403896
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2095-1779 (Print)
IS  - 2214-0883 (Electronic)
IS  - 2214-0883 (Linking)
VI  - 4
IP  - 5
DP  - 2014 Oct
TI  - Simultaneous determination of three curcuminoids in Curcuma longa L. by high 
      performance liquid chromatography coupled with electrochemical detection.
PG  - 325-330
LID - 10.1016/j.jpha.2013.10.002 [doi]
AB  - A novel method for analysis of three active components curcumin, 
      demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by 
      HPLC coupled with electrochemical detection. Three curcuminoids were well 
      separated on a C(18) column and detected with high sensitivity. A mobile phase 
      containing acetonitrile and 10 mM Na(2)HPO(4)-H(3)PO(4) (pH 5.0) (50:50, v/v) was 
      used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 
      0.227-114 muM for curcumin, demethoxycurcumin and bisdemethoxycurcumin 
      respectively. The limit of detection reached up to 10(-8) M, which was lower than 
      that by UV detection. The relative standard deviations (RSDs) ranged from 1.06% 
      to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision, 
      respectively. The proposed method has been applied in real herb sample and 
      recoveries ranging from 86.3% to 111% were obtained.
FAU - Long, Yuling
AU  - Long Y
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan 
      University, Ministry of Education, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan 430071, China.
FAU - Zhang, Wenpeng
AU  - Zhang W
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan 
      University, Ministry of Education, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan 430071, China.
FAU - Wang, Fang
AU  - Wang F
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan 
      University, Ministry of Education, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan 430071, China.
FAU - Chen, Zilin
AU  - Chen Z
AD  - Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan 
      University, Ministry of Education, and Wuhan University School of Pharmaceutical 
      Sciences, Wuhan 430071, China.
LA  - eng
PT  - Journal Article
DEP - 20131105
PL  - China
TA  - J Pharm Anal
JT  - Journal of pharmaceutical analysis
JID - 101579451
PMC - PMC5761211
OTO - NOTNLM
OT  - Curcuma longa L.
OT  - Curcumin
OT  - Electrochemical detection
OT  - High performance liquid chromatography
EDAT- 2014/10/01 00:00
MHDA- 2014/10/01 00:01
PMCR- 2013/11/05
CRDT- 2018/02/07 06:00
PHST- 2013/07/14 00:00 [received]
PHST- 2013/10/29 00:00 [accepted]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2014/10/01 00:00 [pubmed]
PHST- 2014/10/01 00:01 [medline]
PHST- 2013/11/05 00:00 [pmc-release]
AID - S2095-1779(13)00109-3 [pii]
AID - 10.1016/j.jpha.2013.10.002 [doi]
PST - ppublish
SO  - J Pharm Anal. 2014 Oct;4(5):325-330. doi: 10.1016/j.jpha.2013.10.002. Epub 2013 
      Nov 5.