PMID- 29404001
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2095-1779 (Print)
IS  - 2214-0883 (Electronic)
IS  - 2214-0883 (Linking)
VI  - 6
IP  - 5
DP  - 2016 Oct
TI  - Quantification of neomangiferin in rat plasma by liquid chromatography-tandem 
      mass spectrometry and its application to bioavailability study.
PG  - 335-340
LID - 10.1016/j.jpha.2016.03.005 [doi]
AB  - Neomangiferin, a natural C-glucosyl xanthone, has recently received a great deal 
      of attention due to its multiple biological activities. In this study, a rapid 
      and sensitive ultra-high performance liquid chromatography tandem mass 
      spectrometry (UHPLC-MS/MS) method for the quantification of neomangiferin in rat 
      plasma was developed. Using chloramphenicol as an internal standard (IS), plasma 
      samples were subjected to a direct protein precipitation process using methanol 
      (containing 0.05% formic acid). Quantification was performed by multiple 
      reactions monitoring (MRM) method, with the transitions of the parent ions to the 
      product ions of m/z 583.1-->330.9 for NG and m/z 321.1-->151.9 for IS. The assay was 
      shown to be linear over the range of 0.2-400 ng/mL, with a lower limit of 
      quantification of 0.2 ng/mL. Mean recovery of neomangiferin in plasma was in the 
      range of 97.76%-101.94%. Relative standard deviations (RSDs) of intra-day and 
      inter-day precision were both <10%. The accuracy of the method ranged from 94.20% 
      to 108.72%. This method was successfully applied to pharmacokinetic study of 
      neomangiferin after intravenous (2 mg/kg) and intragastric (10 mg/kg) 
      administration for the first time. The oral absolute bioavailability of 
      neomangiferin was estimated to be 0.53%+/-0.08% with an elimination half-life 
      (t(1/2)) value of 2.74+/-0.92 h, indicating its poor absorption and/or strong 
      metabolism in vivo.
FAU - Yang, Bo
AU  - Yang B
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Liu, Zhirui
AU  - Liu Z
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Shang, Shenglan
AU  - Shang S
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Qin, Xiaojian
AU  - Qin X
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
FAU - Xia, Peiyuan
AU  - Xia P
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, PR China.
LA  - eng
PT  - Journal Article
DEP - 20160328
PL  - China
TA  - J Pharm Anal
JT  - Journal of pharmaceutical analysis
JID - 101579451
PMC - PMC5762620
OTO - NOTNLM
OT  - Bioavailability
OT  - Neomangiferin
OT  - Pharmacokinetics
OT  - Rat plasma
OT  - UHPLC-MS/MS
EDAT- 2016/10/01 00:00
MHDA- 2016/10/01 00:01
PMCR- 2016/03/28
CRDT- 2018/02/07 06:00
PHST- 2015/11/11 00:00 [received]
PHST- 2016/03/20 00:00 [revised]
PHST- 2016/03/25 00:00 [accepted]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2016/10/01 00:00 [pubmed]
PHST- 2016/10/01 00:01 [medline]
PHST- 2016/03/28 00:00 [pmc-release]
AID - S2095-1779(16)30025-9 [pii]
AID - 10.1016/j.jpha.2016.03.005 [doi]
PST - ppublish
SO  - J Pharm Anal. 2016 Oct;6(5):335-340. doi: 10.1016/j.jpha.2016.03.005. Epub 2016 
      Mar 28.