PMID- 29404144
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2148-7197 (Print)
IS  - 2149-2530 (Electronic)
IS  - 2148-7197 (Linking)
VI  - 17
IP  - 4
DP  - 2016 Oct
TI  - Analysis of Chromosome 3, 7 and 8 Centromeric Regions in Bronchial Lavage 
      Specimens by FISH.
PG  - 141-147
LID - 10.5152/TurkThoracJ.2016.001 [doi]
AB  - OBJECTIVES: Multiple genetic changes are observed in malignant tumors but are 
      rare or absent in benign conditions. Aneuploidy is the most common feature of 
      solid tumors including lung cancer and diagnosis of malignant tumors is possible 
      through detection of aneuploidy. The aim of this study was to investigate 
      chromosomal abnormalities in cells from non-small cell lung cancer patients 
      obtained bronchoscopically and to evaluate the suitability of fluorescence in 
      situ hybridization (FISH). MATERIAL AND METHODS: Bronchial lavage samples of 17 
      non-small cell lung cancer (NSCLC) patients were evaluated with four-color FISH 
      using deoxyribonucleic acid (DNA) probes specific for the centromere regions of 
      chromosomes 3, 7 and 8. tested specimens were first hybridized with probes, then 
      visualized under fluorescence microscobe and captured with device's camera. 
      RESULTS: High number of aneuploidic cells were detected in all the samples. 
      Increased or decreased abnormal copies or chromosomes 3, 7 and 8 were obserced in 
      all the 17 patients. Aneuploidy of chromosome 3 (21.35%) was higher than those of 
      chromosome 7 (9.06%) and chromosome 8 (15.47%). Moreover, our results were 
      significant for monosomy and trisomy of chromosome 3, trisomy of chromosome 7, 
      nullisomy, monosomy and trisomy of, chromosome 8 (p< 0.05). CONCLUSION: It has 
      been observed that FISH is a useful technique for detection of aneuploidy in 
      bronchial lavage samples obtained by bronchoscopy. Interphase cells were 
      evaluated without cell culturing with this method and high number of tumor cells 
      were enumerated rapidly. Our study has demonstrated that, FISH technique may be 
      used successfully in detection of chromosome number abnormalities in NSCLC 
      patients and may facilitate evaluation of genetic abnormalities.
FAU - Atasoy, Sezen
AU  - Atasoy S
AD  - Department of Medical Biology, Istanbul University Cerrahpasa Faculty of 
      Medicine, Istanbul, Turkey.
FAU - Erturan, Salih Serdar
AU  - Erturan SS
AD  - Department of Chest Diseases, Istanbul University Cerrahpasa Faculty of Medicine, 
      Istanbul, Turkey.
FAU - Yilmaz, Nail
AU  - Yilmaz N
AD  - Department of Chest Diseases, Istanbul University Cerrahpasa Faculty of Medicine, 
      Istanbul, Turkey.
FAU - Kuru, Dilhan
AU  - Kuru D
AD  - Department of Medical Biology, Istanbul University Cerrahpasa Faculty of 
      Medicine, Istanbul, Turkey.
FAU - Cirakoglu, Ayse
AU  - Cirakoglu A
AD  - Department of Medical Biology, Istanbul University Cerrahpasa Faculty of 
      Medicine, Istanbul, Turkey.
FAU - Yilmaz, Sukriye
AU  - Yilmaz S
AD  - Department of Medical Biology, Istanbul University Cerrahpasa Faculty of 
      Medicine, Istanbul, Turkey.
FAU - Deviren, Ayhan
AU  - Deviren A
AD  - Department of Medical Biology, Istanbul University Cerrahpasa Faculty of 
      Medicine, Istanbul, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20161001
PL  - Turkey
TA  - Turk Thorac J
JT  - Turkish thoracic journal
JID - 101648545
PMC - PMC5783093
OTO - NOTNLM
OT  - Lung cancer
OT  - aneuploidy
OT  - bronchial lavage
OT  - cytogenetic
OT  - fluorescence in situ hybridization
COIS- Conflict of Interest: No conflict of interest was declared by the authors.
EDAT- 2016/10/01 00:00
MHDA- 2016/10/01 00:01
PMCR- 2016/10/01
CRDT- 2018/02/07 06:00
PHST- 2015/09/21 00:00 [received]
PHST- 2016/02/15 00:00 [accepted]
PHST- 2018/02/07 06:00 [entrez]
PHST- 2016/10/01 00:00 [pubmed]
PHST- 2016/10/01 00:01 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - ttj-17-4-141 [pii]
AID - 10.5152/TurkThoracJ.2016.001 [doi]
PST - ppublish
SO  - Turk Thorac J. 2016 Oct;17(4):141-147. doi: 10.5152/TurkThoracJ.2016.001. Epub 
      2016 Oct 1.