PMID- 29405213
OWN - NLM
STAT- MEDLINE
DCOM- 20190104
LR  - 20190104
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 143
IP  - 5
DP  - 2018 Feb 26
TI  - Highly sensitive colorimetric detection of allergies based on an immunoassay 
      using peroxidase-mimicking nanozymes.
PG  - 1182-1187
LID - 10.1039/c7an01866e [doi]
AB  - Nanomaterials that exhibit enzyme-like characteristics, which are called 
      nanozymes, have recently attracted significant attention due to their potential 
      to overcome the intrinsic limitations of natural enzymes, such as low stability 
      and relatively high cost for preparation and purification. In this study, we 
      report a highly efficient colorimetric allergy detection system based on an 
      immunoassay utilizing the peroxidase-mimicking activity of hierarchically 
      structured platinum nanoparticles (H-Pt NPs). The H-Pt NPs had a diameter of 30 
      nm, and were synthesized by a seed-mediated growth method, which led to a 
      significant amount of peroxidase-like activity. This activity mainly occurs 
      because of the high catalytic power of the Pt element, and the fact that the H-Pt 
      NPs have a large surface area available for catalytic events. The H-Pt NPs were 
      conjugated to an antibody for the detection of immunoglobulin E (IgE) in the 
      analytes; IgE is a representative marker for the diagnosis of allergies. They 
      were then successfully integrated into a conventionally used allergy diagnostic 
      test, the ImmunoCAP diagnostic test, as a replacement for natural signaling 
      enzymes. Using this strategy, total and specific IgE levels were detected within 
      5 min at room temperature, with high specificity and sensitivity. The practical 
      utility of the immunoassay was also successfully verified by correctly 
      determining the levels of both total and specific IgE in real human serum samples 
      with high precision and reproducibility. The present H-Pt NP-based immunoassay 
      system would serve as a platform for rapid, robust, and convenient analysis of 
      IgE, and can be extended to the construction of diagnostic systems for a variety 
      of clinically important target molecules.
FAU - Cho, Seongyeon
AU  - Cho S
AD  - Department of BioNano Technology, Gachon University, Seongnam, Gyeonggi 13120, 
      Republic of Korea. moonil@gachon.ac.kr.
FAU - Lee, Sang Min
AU  - Lee SM
FAU - Shin, Ho Yun
AU  - Shin HY
FAU - Kim, Min Su
AU  - Kim MS
FAU - Seo, Yiel Hea
AU  - Seo YH
FAU - Cho, Yong Kyun
AU  - Cho YK
FAU - Lee, Jinwoo
AU  - Lee J
FAU - Lee, Sang Pyo
AU  - Lee SP
FAU - Kim, Moon Il
AU  - Kim MI
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 49DFR088MY (Platinum)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - *Colorimetry
MH  - Humans
MH  - *Immunoassay
MH  - Immunoglobulin E/blood
MH  - Metal Nanoparticles/*chemistry
MH  - Peroxidases
MH  - *Platinum
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2018/02/07 06:00
MHDA- 2019/01/05 06:00
CRDT- 2018/02/07 06:00
PHST- 2018/02/07 06:00 [pubmed]
PHST- 2019/01/05 06:00 [medline]
PHST- 2018/02/07 06:00 [entrez]
AID - 10.1039/c7an01866e [doi]
PST - ppublish
SO  - Analyst. 2018 Feb 26;143(5):1182-1187. doi: 10.1039/c7an01866e.